家族性腺瘤性息肉病永生细胞株的建立及意义
Establishment and significance of immortal lymphoblastoid cell lines of familial adenomatous polyposis
王利新 1魏军 1杨宝珍 1潘月英 1张东 2师新荣2
作者信息
- 1. 宁夏医科大学附属医院,医学实验中心,宁夏,银川,750004
- 2. 宁夏医科大学附属医院,结直肠外科,宁夏,银川,750004
- 折叠
摘要
目的:建立家族性腺瘤性息肉病(FAP)家系永生细胞株,为FAP的深入研究提供永久的研究材料. 方法:应用EB病毒(EBV)加入环孢霉素A转化技术,将FAP家系成员外周血B淋巴细胞转化成永生化淋巴母细胞系. 结果:成功为两个FAP家系25个个体建立了永生细胞株,转化率为100%.对已建株保存的永生细胞进行复苏培养,复苏成功率为100%. 结论:FAP永生细胞株保存了原来个体完整的基因组,为在分子水平上揭示FAP的发病机制提供了宝贵的材料.
Abstract
AIM: To establish immortal lymphoblastoid cell lines of familial adenomatous polyposis (FAP) and to provide materials for further research of FAP. METHODS: Immortal lymphoblastoid cell lines of FAP were established by Epstein-Barr (EB) virus transformation of peripheral blood B lymphocytes from members of FAP and addition of cyclosporine A. RESULTS: Twenty-five immortal cell lines of two FAP pedigrees were successfully obtained with a transformation rate of 100%. The culture recovery rate was 100%. CONCLUSION: The established immortal lymphoblastoid cell lines are good materials used for molecular level study of FAP.
关键词
家族性腺瘤性息肉病/疱疹病毒4型人/永生化淋巴母细胞系Key words
familial adenomatous polyposis/herpes virus 4, human/lymphoblastoid cell lines引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据