苦参碱诱导U937细胞分化及其相关机制
Differentiation of U937 cells induced by Matrine and its mechanism
卢前微 1陈建斌 1汤为学 2杨泽松 1葛群芳 1廖洋1
作者信息
- 1. 重庆医科大学,附属第一医院血液内科,重庆,400016
- 2. 重庆医科大学,病理生理学教研室,重庆,400016
- 折叠
摘要
目的:观察苦参碱对U937细胞诱导分化作用,并探讨其可能作用机制. 方法:采用流式细胞仪检测细胞周期分布;硝基四氮唑蓝(NBT)还原实验、CD11b,CD14表面抗原的表达及细胞形态学检测细胞分化;Western Blot检测p21Wafl/Cipl的蛋白表达. 结果:苦参碱作用U937细胞后,细胞周期分析提示发生S期阻滞;NBT阳性细胞率明显增高(P<0.05),CD11b表达增加(P<0.05);细胞内p21Wafl/Cipl表达增高(P<0.05). 结论:苦参碱能诱导U937细胞分化,其机制可能与细胞周期调控蛋白p21Waf1/Cip1表达的改变有关.
Abstract
AIM: To investigate the effects of Matrine on the differentiation in U937 cells and its mechanism. METHODS: The distribution of cell cycle was measured by flow cytometry. Cell differentiation was detected by NBT reduction test, expression of cell surface markers (CD11b and CD14) and morphologic observation. Western blot was used to detect the protein expression of p21Waf1/Cip1. RESULTS: Cell-cycle analyses of the Matrine-treated cells indicated that the cells were arrested in S-phase. The NBT positive cells and the expression of CD11b increased obviously (P ＜ 0.05). The expression of p21Waf1/ Cip1 was significantly up-regulated in Marrine-treated U937 cells (P ＜ 0.05). CONCLUSION: Matrine induces the differentiation of U937 cells, which may be related to the changes of p21Waf1/Cip1.
关键词
苦参碱/U937细胞/细胞分化/细胞周期/p21Waf1/Cip1Key words
matrine/U937 cells/cell differentiation/cell cycle/p21Waf1/Cip1引用本文复制引用
基金项目
重庆市卫生局科研基金课题(07-2-035)
重庆市教育委员会科学技术研究项目(KJ08032i)
重庆医科大学创新基金(XBYB2007024)
出版年
2009
NETL
NSTL
维普
万方数据