Effect of Sanjiayi Gan granula contained serum on TGF-β1, TIMP-1 gene expression of HSC-T6 cells
AIM: To observe the in vitro effect of Sanjiayi Gan granula (S-JG) contained serum on TGF-β1 mRNA, TIMP-1 mRNA expression of HSC-T6 cells. METHODS: HSC-T6 cells were exposed in different doses of Sanjiayi gan granula contained serum for 48 h. TGF-β1 mRNA, TIMP-1 mRNA levels were measured by quantitative reverse-transcription polymerase chain reaction (RT-PCR). RESULTS: TGF-β1 mRNA, TIMP-1 mRNA levels of HSC-T6 cells at different doses of S-JG contained serum were 1.65 ± 0.05, 1.67 ± 0.04, 1.23 ± 0.07, 0.72 ± 0.02 and 2.23 ± 0.12, 2.17 ± 0.12, 1.41 ± 0.03, 0.49 ± 0.09, respectively, which were lower than those of normal control (2.11 ± 0.11, 2.72 ± 0.11, P ＜ 0.05, respectively). CONCLUSION: Sanjiayi gan granula (S-JG) contained serum can decrease TGF-β1 mRNA, TIMP-1 mRNA expression of HSC-T6 cells.
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