Effect of oleanolic acid on proliferation and cell cycle block of human lung adenoma A549
AIM: To study the effects of oleanolic acid (OA) on the cell proliferation and the cycle block in human lung adenoma cell line A549. METHODS: A549 cells were incubated in vitro and the mice were assigned to groups with OA concentration of 1.25, 2.5, 5, 10, 20 and 40 mg/L. The cell proliferation and the proliferation inhibition rate were detected by MTT assay at 24, 48 and 72 h. The distribution of cells in cycle was assessed by flow cytometry (FCM) at 24 h. RESULTS: The proliferation inhibition rates of A549 rose with the increase of OA concentration and time length(P＜0.05). At 24h, the cell ratio of the cells in G0/G1 cycles was higher in 10, 20 and 40 mg/L groups compared with that in control. The cell ratio increased in a concentration-effect dependent manner (P ＜ 0.01) and the ratio rose with the increase of OA concentration. CONCLUSION: OA exerts activated effects on anti-lung adenoma cells in a time and dosage dependent manner and inhibits the proliferation of cancer cells possibly as a result of G1 cycle block in the cells.
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万方数据
维普
