谷胺酰胺预处理对兔脊髓缺血再灌注损伤后血小板聚集及活化功能的影响
Effect of glutamine precondition on platelet aggregation and activation functions in rabbits with spinal cord ischemia/reperfusion injury
巩守平 1王文涛 1钟大林 1吕健 1吴锋 2车金 2僧志远 1贺西京3
作者信息
- 1. 西安交通大学医学院,第二附属医院神经外科,陕西,西安,710004
- 2. 西安交通大学医学院,血液病实验室,陕西,西安,710004
- 3. 西安交通大学医学院,第二附属医院骨科,陕西,西安,710004
- 折叠
摘要
目的:探讨血小板聚集及活化功能在脊髓缺血再灌注损伤(SCIRI)中的变化及谷氨酰胺预处理对其的影响. 方法:利用Zivin法建立家兔SCIRI模型,动态监测SCIRl及谷氨酰胺预处理时血小板聚集试验(PAgT)及活化功能特异指标血浆GMP-140浓度的变化. 结果:PAgT及GMP-140浓度在缺血45 min逐渐升高,持续至再灌注6 h,与Sham组相比差异显著(P<0.01),于再灌注12 h下降到缺血前水平(P>0.05);谷氨酰胺预处理后血小板进一步活化,高峰期延迟. 结论:血小板处于过度活化状态,聚集功能增强.引起凝血功能亢进,可能是参与了脊髓缺血再灌注后神经元损伤与修复过程,谷氨酰胺预处理后血小板进一步活化.
Abstract
AIM: To explore the effect of glutamine precondition on platelet aggregation and activation functions in rats with spinal cord ischemia/reperfusion injury (SCIRI). METHODS: SCIRI models were established with Zivin method in rabbits. The platelet aggregation Test(PAgT) and the plasma GMP-140 were observed dynamically. RESULTS: PAgT and GMP-140 concentration in the IR group started to increase at 45 min after ischemia and continued to increased until 6 h after reperfusion, and then decreased to the level before ischemia at 12 h after reperfusion, Glutamine precondition delayed the peak of platelet activation. CONCLUSION: Enhanced PAgT and excessively activated blood platelet function may be involved in the pathological process of SCIRI, which can be activated by glutamine preconditioning.
关键词
缺血再灌注损伤/脊髓/PAgT/GMP-140/谷氨酰胺Key words
ischemia/reperfusion/spinal cord/PAgT/GMP-140/glutamine引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据