Effect of siRNA target gene CXCR4 on invasion capability of EC9706 cells
AIM: To explore the effect of silencing CXCR4 by siRNA on the invasion capability of esophageal carcinoma cell line EC9706 in vitro. METHODS: Two siRNAs targeting CXCR4 and one fluorescence-labeled siRNA as a negative control were chemically synthesized and were transfected into EC9706 cells. The transfection efficiency was observed under fluorescence microscope, CXCR4 mRNA and protein levels were detected by semi-quantitative RT-PCR and Western blot after 48 h, and the invasion capability of EC9706 cells in vitro was evaluated by Boyden Chamber. RESULTS: The two sequence-specific siRNAs targeting CXCR4 efficiently suppressed the expression of CXCR4 at mRNA and protein levels in EC9706 cells compared with control groups after 48 h (P ＜ 0.05). The expression of CXCR4 in EC9706 cells transfected with CXCR4 siRNAl decreased more obviously, but the expression of CXCR4 with no transfection was not significantly different from that in control groups (P ＞ 0.05). The results of Boyden Chamber experiment showed that the number of cells under micro-membrane decreased in EC9706 cells transfected with CXCR4 two sequence-specific siRNAs after 48 h compared with those in the control group (P ＜ 0.05), while no significant difference was observed between control groups (P ＞ 0.05). CONCLUSION: Silencing CXCR4 by siRNA decreases the expression of CXCR4 and the invasion capability of EC9706 cells. CXCR4 gene may be a potentially valuable therapeutic target in the treatment of esophageal carcinoma.
NETL
NSTL
万方数据
维普
