AFP和IL-18双表达腺病毒转染DC肝癌瘤苗的制备及表达
Construction of ILI8-AFP-DC vaccine mediated by IL-18 and AFP gene coexpressing adenovirus and detection of its expression
张吉成 1王弼 1贾军 2封江南 3陈涛 1陈燕凌1
作者信息
- 1. 福建医科大学附属协和医院肝胆外科,福建,福州,350001
- 2. 北京大学临床肿瘤学院,北京肿瘤医院暨北京市肿瘤防治研究所内科,北京100142
- 3. 武汉市晶赛生物工程技术有限公司,湖北,武汉,430074
- 折叠
摘要
目的:制备IL-18基因修饰的树突状细胞AFP瘤苗,探索增强肝癌树突状细胞免疫治疗效果的新途径. 方法:构建和制备rAd-IL18-AFP双基因表达的重组腺病毒,转染DC,应用RT-PCR和Western Blot方法检测IL-18和AFP基因在DC中的蛋白表达. 结果:成功制备IL-18基因修饰的树突状细胞AFP瘤苗,rAd-IL18-AFP介导的IL-18和AFP基因能够在DC中进行蛋白表达. 结论:成功制备IL-18基因修饰的树突状细胞AFP瘤苗,为进一步的肝癌免疫基凶治疗研究打下基础.
Abstract
AIM: To construct the IL18-AFP-DC vaccine mediated by IL-18 and AFP gene coexpressing adenovirus and to explore a new method of improving the immune response against hepatocellular carcinoma. METHODS: Cytokine interleukin-18 and AFP gene were transfected into dendritic cells (DC) mediated by adenovirus and the expressions of cytokine interleukin-18 and AFP gene in DC were detected by RT-PCR and Western blot. RESULTS: The IL18-DC-AFP vaccine was successful constructed. CONCLUSION: The IL18-AFP-DC vaccine mediated by IL-18 and AFP gene coexpressing adenovirns is successfully constructed, which has laid the basis for the downstream study of improving immune response against hepatocellular carcinoma.
关键词
白细胞介素18/树突状细胞瘤苗/甲胎蛋白/肝癌/基因治疗Key words
interleukin 18/dendritie cell vaccine/alpha-fetoprotein/hepatocellular carcinoma/gene therapy引用本文复制引用
基金项目
福建省自然科学基金(C0540008)
福建医科大学附属协和医院博士科研启动基金(院管基金)()
出版年
2009
NETL
NSTL
维普
万方数据