Construction of ILI8-AFP-DC vaccine mediated by IL-18 and AFP gene coexpressing adenovirus and detection of its expression
AIM: To construct the IL18-AFP-DC vaccine mediated by IL-18 and AFP gene coexpressing adenovirus and to explore a new method of improving the immune response against hepatocellular carcinoma. METHODS: Cytokine interleukin-18 and AFP gene were transfected into dendritic cells (DC) mediated by adenovirus and the expressions of cytokine interleukin-18 and AFP gene in DC were detected by RT-PCR and Western blot. RESULTS: The IL18-DC-AFP vaccine was successful constructed. CONCLUSION: The IL18-AFP-DC vaccine mediated by IL-18 and AFP gene coexpressing adenovirns is successfully constructed, which has laid the basis for the downstream study of improving immune response against hepatocellular carcinoma.
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NSTL
万方数据
维普
