自身免疫性甲状腺病患者外周血T淋巴细胞CTLA4和CD40L的表达
Expression of CTLA-4 and CD40L on periperal T lymphocytes in autoimmune thyroid disease
王宇 1张健 2张进安 1肖婉侠 1全瑛 1刘琳 1王荣1
作者信息
- 1. 西安交通大学医学院第一附属医院,内分泌科,陕西,西安,710061
- 2. 西安交通大学医学院第一附属医院,检验科,陕西,西安,710061
- 折叠
摘要
目的:通过检测自身免疫性甲状腺病(AITD)患者外周血T淋巴细胞表面细胞毒性T淋巴细胞相关抗原-4(CTLA-4,CD152)和CIMO配体(CIMOL,CD154)的表达,阐明共刺激分子在自身免疫性甲状腺病发生中的作用. 方法:对30例Graves病(GD),20例桥本甲状腺炎(HT)患者和20例健康对照者采用流式细胞仪分析了共刺激分子在外周血T淋巴细胞上的表达. 结果:与正常对照相比,外周血T淋巴细胞表达CTLA-4的百分数在GD患者中升高,在HT患者中降低.GD患者表达CTLA-4百分数与TSAb滴度呈正相关. 结论:AITD外周血T淋巴细胞呈现共刺激分子的异常表达,某些分子的表达与自身抗体水平有相关性,提示共刺激分子参与了AITD的免疫发病机制.
Abstract
AIM: To investigate the possible abnormal expression of costimulatory molecules of cytotoxic-T-lymphocyte-associated-antigen-4 (CTLA4, CD152) and CD40 ligand (CD40L, CD154) on peripheral blood T cells in patients with autoimmune thyroid disease (AITD) and to analyze the possible relation between these molecules and clinical indices. METHODS: The expression of costimulatory molecules on peripheral blood CD3+ T lymphocytes was detected by two-color flow cytometry in 30 patients with Graves' disease (GD), 20 patients with Hashimoto's thyroiditis (HT) and 20 healthy controls. RESULTS: Compared with that in healthy controls, the percentage of CTLA-4 expressed CD3+ T lymphocytes was significantly higher in GD patients. But in HT patients, the expression of CTLA-4 significantly decreased. A significant positive correlation was found between CTLA-4 expression and the serum level of TSAb in patients with GD. CONCLUSION: The abnormal expression of CTLA-4 on peripheral blood T lymphocytes suggests that costimulatory molecules may be involved in the pathogenesis of AITD.
关键词
自身免疫/甲状腺病/T淋巴细胞/CTLA-4/CIMOLKey words
autoimmune/thyroid disease/T lymphocyte/CTLA-4/CD40L引用本文复制引用
基金项目
国家自然科学基金(30871185)
陕两省科技攻关项目(2007k12-02)
出版年
2009
NETL
NSTL
万方数据