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HPLC-MS法测定人血浆中依普利酮浓度

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目的:建立人血浆中依普利酮浓度的HPLC-MS测定法. 方法:血浆样品中加入内标溶液后,以液-液萃取方法进行样品处理,采用HPLC-MS方法测定其血药浓度. 结果:标准曲线线性范围为2.080-4160μg/L,标准曲线线性关系良好,回归方程为:f=0.0005520+0.001333×C(r=0.9984).高、中、低3浓度的批内和批间变异均小于15%,提取回收率在73.6%～75.5%. 结论:建立人血浆中依普利酮浓度的HPLC-MS测定法操作简便,灵敏度高,适用于依普利酮的血药浓度检测及药动学研究.
Determination of eplerenone in human plasma by HPLC-MS
AIM: To develop an HPLC-MS method for the determination of Eplerenone concentration in human plasma. METHODS: The samples were extracted with ethyl acetate after adding the internal standard solution, and analytes were detected by mass spectrometer in positive models. RESULTS: The standard curve was linear in the range of 2.080 - 4160 μg/L and the mean calibration curve: f = 0.0005520 + 0.001333 × C (r = 0.9984). The relative standard derivation of intra- and inter-run was all less than 15%. The extraction recoveries of eplerenone were between 73.6% - 75.5%. CONCLUSION: A specific, sensitive and simple method has been established, which is good for determination of eplerenone concentration in human plasma and for pharmacokinetic studies.

eplerenonespectrum analysis, masschromatography, high pressure liquidplasma concentration

赵越平、陈苏宁、丁莉坤、杨志福、王志睿、金鑫、文爱东

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第四军医大学西京医院药剂科,陕西,西安,710033

依普利酮 光谱分析 质量 色谱法 高压液相 血药浓度

2009

第四军医大学学报
第四军医大学

第四军医大学学报

CSTPCDCSCD北大核心
影响因子：0.599
ISSN：1000-2790
年,卷(期)：2009.30(18)
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