AIM: To further observe B-1 B cell function in innate immune system, we compare and analyze CD14 expression on B1 and B2 cells. METHODS: Peritoneal cells and splenocytes of normal C57BL/6 mice were isolated and purified with anti-IgM magnetic beads. The total RNA of the cells were extracted and the expression level of CD14 was detected by semiquantitative RT-PCR. CD14 expression on B1 and B2 cells were measured using anti-CD14 and anti-CD19 antibodies by flow cytometry (FCM). Furthermore, LPS was injected into mice abdominal cavity, then peritoneal cells and splenocytes at different time points were isolated and purified by magnetic beads. The change of CD14 expression level was detected by semi-quantitative RT-PCR. RESULTS: The results of RT-PCR and FCM revealed that CD14 expression level on B1 cell was relatively low. However, almost no CD14 expression was detected on B2 cell. CD14 expression increased significantly after LPS stimulation on B1 cell. CONCLUSION: B1 cell of normal mice expresses CD14, which may play a significant role in the immune responses to LPS.
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NSTL
万方数据
维普
