Fabrication and biocompatibility evalu-ation of a new liver tissue engineering scaffold
AIM: To fabricate a new liver tissue engineering scaffold and evaluate its biocompatibility so as to provide a reference basis to applicate in liver tissue engineering. METHODS: We chose chitosan and gelatin as the scaffold materials, New Rapid Prototyping technology and Microfabrication technology were applied to fabricate the new type liver engineering scaffold. In the following study, we seeded rat primary hepatocyte in the new scaffold, morphological and functional tests were both used to evaluate the new scaffold's biocompatibility. RESULTS: The new liver chitosan/gelatin porous scaffolds we had fabricated had high-porosity and large-surface area, which was favorable to the attachment of hepatocyte. The hepatocytes which were seeded in the scaffold had a good growing and propagation tendency during the culture period. The new scaffold had a slow uniform degradation rate and maintained good 3-D structure. CONCLUSION: The new liver chitosan/gelatin porous scaffolds we had fabricated had suitable biocompatibility, low cytotoxicity and accord with liver tissue engineering standards.
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维普
