首页|EPHB6受体型酪氨酸蛋白激酶对ERK信号通路的调控效应

EPHB6受体型酪氨酸蛋白激酶对ERK信号通路的调控效应

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目的:探讨促红细胞生成素产生肝细胞B6(EPHB6)对于胞外信号调节激酶(ERK)信号转导通路的作用.方法:以非小细胞肺癌细胞系A549作为研究对象,采用Western Blot法检测ERK的磷酸化水平及通路检测(PathDe-tect)法检测ERK下游分子和Ets结构域蛋白1(Elk-1)转录因子的活性.结果:A549细胞中EPHB6的表达导致ERK的磷酸化.相反,siRNA干涉EPHB6的表达可以逆转ERK的磷酸化.并且,EPHB6导致的ERK磷酸化与Elk-1转录因子脱偶联.结论:表明EPHB6对于ERK信号通路具有调控作用.
Regulation of EPHB6 receptor tyrosine kinase on the ERK signaling pathway
AIM:To investigate the regulatory effects of EPHB6 receptor tyrosine kinase on the signaling pathway of ERK MAPK. METHODS:A549 non-small cell lung cancer cell line was used in the experiments. Western blotting was used to assess the phosphorylation levels of EPHB6, and the status of Elk-1, a transcriptional activator downstream of EPHB6, was determined by PathDetect kit. RESULTS: Expression of EPHB6 induced the expression knocking-down by EPHB6 siRNA of ERK in A549 cells, reversed the phosphorylation of ERK. Moreover, EPHB6-induced phosphorylation of ERK was uncoupled with Elk-1 transcriptional activator. CONCLUSION:EPHB6 plays a role in the regulation of ERK signaling pathway.

NSCLC, non-small cell lung cancerMAPK, mitogen-activated protein kinaseERK, extracellular-signal-regulated kinaseDMSO, dimethylsulfoxideFACS, fluorescence-activated cell sorting

铁茹、陈宝莹、刘利兵、陈健康、张学策、周幸春、曲萍、黄小军、于军

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第四军医大学,基础部基础医学教学实验中心,陕西,西安,710033

第四军医大学,唐都医院放射科,陕西,西安,710038

EPHB6 受体型酪氨酸蛋白激酶 非小细胞肺癌 细胞外信号调节激酶 有丝分裂原蛋白活化激酶 Elk-1

国家自然科学基金

30801385

2009

第四军医大学学报
第四军医大学

第四军医大学学报

CSTPCDCSCD北大核心
影响因子：0.599
ISSN：1000-2790
年,卷(期)：2009.30(19)
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