胰岛素样因子3在邻苯二甲酸二(2-乙基)己酯诱导隐睾发生过程中的表达分析
Effect of Di-(2-ethylhexyl) phthalate on insulin like factor 3 expression in fetal mice
吴盛德 1朱静 2李娅莎 2甘立强 1袁心刚 1徐明灯 1魏光辉1
作者信息
- 1. 重庆医科大学,附属儿童医院泌尿外科,重庆,400014
- 2. 重庆医科大学,附属儿科研究所,重庆,400014
- 折叠
摘要
目的:研究环境内分泌干扰物邻苯二甲酸二(2-乙已基)酯(Di-(2-ethylhexyl)phthalate,DEHP)作用于孕鼠后,其胎鼠睾丸组织中胰岛素样因子3(Insulin like factor 3,INSL3)mRNA和蛋白质的表达情况;探讨DEHP诱发小鼠隐睾的分子机制.方法:妊娠KM小鼠随机分为3组:正常对照组、玉米油对照组和DEHP实验组.玉米油对照组和DEHP实验组自妊娠12.5 d到妊娠18 d分别持续经口予以玉米油和DEHP 500 ms/(kg·d),正常对照组不予灌药.结果:正常对照组和玉米油对照组睾丸组织中INSL3 mRNA和蛋白质的表达均无差异(P>0.05);DEHP实验组INSL3 mRNA的表达量约为对照组的1/3,与对照组相比其差异具有显著性(P<0.05);实验组INSL3蛋白质的表达量约为对照组的1/4,与对照组相比其差异具有显著性(P<0.05).结论:环境内分泌干扰物DEHP下调睾丸间质细胞INSL3 mRNA和蛋白质的表达,影响睾丸引带发育,可能是DEHP诱发隐睾的分子机制之一.
Abstract
AIM: To investigate the expression of Insulin like factor 3 (INSL3) in fetal mice exposed to Di-(2-ethylhexyl) phthalate (DEHP) in pregnancy. METHODS: KM mice were divided into 3 groups randomly: normal control group, com oil group and DEHP-exposed group. The last two groups were administrated respectively with corn oil and DEHP 500 mg/(kg · d) to the pregnant mice from gestation day 12.5 (GD12.5) to GD18, and normal group with nothing. RESULTS: Expression of INSL3 mRNA and protein in normal and com group were no significance (P ＞ 0.05). Expression of INSL3 mRNA in DEHP-exposed group was about one-third of that in normal group, which was significantly decreased (P ＜ 0.05). Similarly, expression of INSL3 protein in DEHP-exposed group was about quarter of that in normal group, which was also significantly decreased (P ＜ 0.05). CONCLUSION: DEHP as one of environmental endocrine disrupters, can down-regulate the mRNA and protein expression of INSL3 and interfere the gubemaculum development. It may be a possible mechanism that DEHP causes cryptorchidism.
关键词
环境内分泌干扰物/邻苯二甲酸二(2-乙已基)酯/胰岛素样因子3/隐睾Key words
environmental endocrine disrupters/Di-(2-ethylhexyl) phthalate/insulin like factor 3/cryptorchidism引用本文复制引用
基金项目
国家自然科学基金(30872706)
重庆市自然科学基金(2007BB5325)
出版年
2009
NETL
NSTL
维普
万方数据