Effect of Di-(2-ethylhexyl) phthalate on insulin like factor 3 expression in fetal mice
AIM: To investigate the expression of Insulin like factor 3 (INSL3) in fetal mice exposed to Di-(2-ethylhexyl) phthalate (DEHP) in pregnancy. METHODS: KM mice were divided into 3 groups randomly: normal control group, com oil group and DEHP-exposed group. The last two groups were administrated respectively with corn oil and DEHP 500 mg/(kg · d) to the pregnant mice from gestation day 12.5 (GD12.5) to GD18, and normal group with nothing. RESULTS: Expression of INSL3 mRNA and protein in normal and com group were no significance (P ＞ 0.05). Expression of INSL3 mRNA in DEHP-exposed group was about one-third of that in normal group, which was significantly decreased (P ＜ 0.05). Similarly, expression of INSL3 protein in DEHP-exposed group was about quarter of that in normal group, which was also significantly decreased (P ＜ 0.05). CONCLUSION: DEHP as one of environmental endocrine disrupters, can down-regulate the mRNA and protein expression of INSL3 and interfere the gubemaculum development. It may be a possible mechanism that DEHP causes cryptorchidism.
environmental endocrine disruptersDi-(2-ethylhexyl) phthalateinsulin like factor 3cryptorchidism
NETL
NSTL
万方数据
维普
