Protection of erythropoietin on myocardial ischemia reperfusion injury in rats
AIM:To explore the effect of erythropoietin (EPO) on myocardial ischemia reperfusion injury in rats, discuss the mechanism involved. METHODS: Thirty-five SD rats were randomly divided into 4 groups: Sham operation group (group SHAM), and 3 experimental groups (group IR, group EPO and group EPO + LY). The ischemia repersusion heart model was established by ligating the left anterior descending branch of coronary artery; EPO was administrated by intravenous between ischemia and reperfusion. The occurrence of arrhythmia in electrocardiogram of lead Ⅱ was observed. The change of cardiac ultrastructure were examed by transmission electron microscope. Myocardium cells apoptosis were estimated by TUNEL; bcl-2, bax and caspase3 mRNA transcription were detected by RT-PCR. RESULTS: EPO could lighten the destruction of cardic ultrastructure, lighten reperfusion arrhythmia; EPO could up-regulate the mRNA transcription of bcl-2, and down-regulate the mRNA transcription of bax and caspase-3, inhibit the apoptosis myocardium cells; LY294002 attenuated the effect of EPO. CONCLUSION: EPO could attenuate myocardial ischemia-reperfusion injury. The cardioprotection by EPO required the PI3K pathway.
NETL
NSTL
万方数据
维普
