Effect of limb immobilization on insulin sensitivity in rats
AIM: To observe the effects of limb immobilization on the insulin sensitivity. METHODS: Sprague-Dawley rats were randomly assigned to 2 groups: control (CON) and three-week immobilization (IB). The right legs of the rats in IB group were immobile for three weeks. After the 3 weeks, hyperinsulinem ic-euglycemic clamp was used to evaluate the insulin sensitivity and soleus muscles were dissected and weighted. The diameter and cross section area of muscle cell were measured. RESULTS: In IB group, the wet weights of the soleus [(169.2 ± 4.1)g vs (67.1 ± 6.3 )g], the diameter of the muscle cell [(49.8 ± 2.7) μm vs (20.6 ± 3.2) μm)] and the area of its cross section decreased [(998 ± 21) μm2 vs (579 ± 14) μm2)] obviously as compared with those in CON group. The glucose infusion rate was significant lower than that in CON group [(35 ± 5) mg/(kg · h) vs (13 ± 4)mg/(kg · h)]. CONCLUSION: Limb immobilization induces soleus atrophy and decreases the insulin sensitivity.
insulin sensitivitehyperinsulinem ic-euglycem ic clampImmobilizationsoleus atrophy
NETL
NSTL
万方数据
维普
