Effect of EGb761 on expression of p75NTR in cultured hippocampal neurons after glutamate -induced excitotoxicity
AIM: To observe the changes in the expression level of p75NTR(p75 neurotrophin receptor) in cultured hippocampal neurons after glutamate-induced excitotoxicity and to explore the correlation between the antiexcitotoxic effect of Ginkgo biloba extract(EGb761) and p75NTR. METHODS: Excitotoxicity was induced by glutamate in rat cultured hippocampal neurons and the neuroprotective effect of 25 - 200 mg/L EGb761 was observed. RT-PCR, Western blot and immunofluorescence staining were used to detect the changes in the expression level of p75NTR. RESULTS: 100 mg/L was determined as the optimal dose of EGb761 against glutamate-induced excitotoxicity. p75NTR at mRNA and protein levels increased markedly compared with those in control group(P ＜ 0.01). EGb761 itself had no obvious effect on p75NTR expression, but EGb761 pretreatment decreased p75NTR at mRNA and protein levels dramatically compared with that in glutamate group (P ＜ 0.05). CONCLUSION: p75NTR plays an important role in neuronal damage after glutamate-induced excitotoxicity and the antiexcitotoxic effect of EGb761 might relate to reduction of the expression level of p75NTR.
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万方数据
维普
