EGb761对谷氨酸诱导海马神经元兴奋毒性损伤中p75NTR表达的影响
Effect of EGb761 on expression of p75NTR in cultured hippocampal neurons after glutamate -induced excitotoxicity
吴兰香 1滕永真 1李春莉 1吴明军1
作者信息
- 1. 重庆医科大学生命科学研究院,重庆,400036
- 折叠
摘要
目的:观察谷氨酸诱导海马神经元兴奋毒性损伤中神经营养因子受体p75(p75 neurotrophin receptor,p75NTR)表达水平的变化,探讨银杏叶提取物(EGb761)抗兴奋毒性神经保护作用与p75NTR的相关性.方法:建立谷氨酸诱导的新生大鼠海马神经元兴奋毒性损伤模型,观察25～200 ms/L剂量下EGb761的神经保护作用;RT-PCR,Western Blot及细胞免疫荧光染色方法检测p75NTR的表达变化.结果:100 mg/LEGb761预处理给药的神经保护效果最明显;谷氨酸刺激后,p75NTR在mRNA和蛋白质水平的表达较对照组明显增高(P<0.01);EGb761本身对p75NTR表达无影响,而EGb761预处理组p75NTR在mRNA和蛋白质水平的表达较谷氨酸组均显著降低(P<0.05).结论:p75NTR在谷氨酸诱导兴奋毒性神经损伤中起着重要作用,EGb761抗兴奋毒性神经保护作用可能与抑制p75NTR的表达有关.
Abstract
AIM: To observe the changes in the expression level of p75NTR(p75 neurotrophin receptor) in cultured hippocampal neurons after glutamate-induced excitotoxicity and to explore the correlation between the antiexcitotoxic effect of Ginkgo biloba extract(EGb761) and p75NTR. METHODS: Excitotoxicity was induced by glutamate in rat cultured hippocampal neurons and the neuroprotective effect of 25 - 200 mg/L EGb761 was observed. RT-PCR, Western blot and immunofluorescence staining were used to detect the changes in the expression level of p75NTR. RESULTS: 100 mg/L was determined as the optimal dose of EGb761 against glutamate-induced excitotoxicity. p75NTR at mRNA and protein levels increased markedly compared with those in control group(P ＜ 0.01). EGb761 itself had no obvious effect on p75NTR expression, but EGb761 pretreatment decreased p75NTR at mRNA and protein levels dramatically compared with that in glutamate group (P ＜ 0.05). CONCLUSION: p75NTR plays an important role in neuronal damage after glutamate-induced excitotoxicity and the antiexcitotoxic effect of EGb761 might relate to reduction of the expression level of p75NTR.
关键词
EGb761/谷氨酸/兴奋毒性/p75NTR/神经保护Key words
EGb761/glutamate/excitotoxicity/p75NTR/neuroprotection引用本文复制引用
基金项目
重庆医科大学校级科研项目(0200101097)
出版年
2009
NETL
NSTL
维普
万方数据