Effect of Bufalin on rat's glomerular mesangial cell proliferation and extra-cellular matrix hyperplasia
AIM: To investigate the effect of Bufalin on rat's glomerular mesangial cells (GMC) proliferation and hyperplastic extracellular matrix (EMC) induced by lipopolysaccharide (LPS). METHODS: Rat's GMC lines were cultivated. GMCs in vitro were divided into 3 groups: control group, LPS group (LPS 5 mg/L) and Bufalin group (LPS 5 mg/L and Bufalin 1 × 10-8 mol/L). The proliferation of GMC was assessed by MTT assay and flow cytometry. mRNA of transforming growth factor β1 (TGF-β1) was measured by reverse transcriptase polymerase chain reaction (RT-PCR) and the expression of Fibronectin (FN) and TGF-β1 in the conditioned medium was measured by enzyme linked immunosorbent assay (ELISA). RESULTS: The GMC proliferation was induced in LPS group, but it was inhibited in Bufalin group in a dose-dependent manner. The percentage of cells in S phase in Bufalin group was significantly lower than that in LPS group (P ＜ 0.01). The expression of TGF-β1 mRNA was significantly inhibited in Bufalin group (P ＜ 0.05). The expressions of FN and TGF-β1 protein in Bufalin group were significantly less than those in LPS group (P ＜ 0.01). CONCLUSION: Bufalin inhibits the GMC proliferation and LPA-induced hyperplastic ECM components.
NETL
NSTL
万方数据
维普
