K-阿片受体激动剂U50488H对大鼠心肌缺血再灌注损伤的直接保护作用
Direct protectory effects of K-opioid receptor agonist U50488H on myocar-dial ischemia- reperfusion in rats
徐庆杰 1李培杰 2张正义 2秦静 2王映珍 2孙志江 2昝香艺2
作者信息
- 1. 北京老年医院,北京,100095
- 2. 兰州大学第二医院急诊内科,甘肃,兰州,730000
- 折叠
摘要
目的:探讨选择性K-阿片受体激动剂U50488H对大鼠心肌缺血再灌注(I/R)损伤冠状微循环和心肌超微结构的影响,明确其对I/R心肌是否具有直接保护作用.方法:将32只大鼠随机分为4组,即假手术组,I/R组,U50488H+I/R组和Nor-BNI(特异性阿片受体阻断剂)+U51M88H+I/R组,每组8只.实验组通过开胸结扎冠脉前降支制备大鼠I/R模型,观察各组大鼠心肌超微结构和冠状微循环的改变.结果:①U50488H+I/R组与I/R组和Nor-BNI+U50488H+I/R组比较,微血管显著扩张(P<0.05),毛细血管腔内血细胞明显减少,心肌间质中偶见红细胞.②U50488H+I/R组与UR组和Nor-BNI+US0488H+I/R组比较,心肌超微结构损伤明显减轻.结论:US0488H可通过激活心脏K-阿片受体,明显改善大鼠L/R心肌的微循环,减轻心肌超微结构的损伤,从而发挥对心脏的直接保护作用,该作用可被选择性的K-阿片受体阻断剂Nor-BNI所拮抗.
Abstract
AIM: To study the effects of U50488H (a selective κ-opioid receptor agonist) on the coronary microcirculation and myocardial ultra structure during myocardial ischemia and reperfusion in rats so as to know whether has some direct protective effects. METHODS: Rats were randomly divided into 4 different groups: control group, ischemia-reperfusion group (I/R), U50488H + I/R group, and Nor-BNI + U50488H + I/R group (Nor-BNI, a selective κ-opioid receptor antagonist). The coronary microcirculation and the myocardial ultra structure were determined in rats. RESULTS: The section area of blood capillary in U50488H + I/R group was larger than that in I/R group and Nor-BNI + U50488H + I/R group. The injury of the myocardial ultra structure was significantly more decreased compared with that in I/R group and Nor-BNI + U50488H + I/R group. CONCLUSION: U50488H improves the microcirculation and decreases the injury of myocardial ultra structure through stimulating K-opioid during myocardial ischemia and reperfusion in rats. U50488H has some direct protective effects during myocardial ischemia and reperfusion in rats, but the effect of U50488H can be abolished by Nor-BNI.
关键词
U50488H/K-阿片受体/心肌缺血-再灌注/微循环/超微结构Key words
U50488H/κ-opioid receptor/myocardial ischemia-reperfusion/microcirculation/infarction ultra structure引用本文复制引用
基金项目
甘肃省科学事业费研究项目(QS061-C33-38)
出版年
2009
NETL
NSTL
万方数据