Vascularization in rat tissue engineering bladder with scaffold of small intestinal submucosa implanted with VEGF165 transplanted bladder muscular cells
AIM: To understand the role of vascularization induced by VEGF165 transinfected bladder muscular cells in rat tissue engineering bladder with scaffold of small intestinal submucosa(SIS). METHODS: The rat's bladders were augumentated with scaffold of small intestinal submucosa (SIS) implanted with VEGF165 transinfected bladder muscular cells. At the same time, another two groups of rats augumentated with SIS without transinfected cell or partial bladder mucosa and smooth muscle destroyed were used as controls. Samples were taken at regular intervals, and were detected with histological and immunohistochemistry staining against CD34 and VEGFR2 expression. RESULTS: In the control group without SIS transplantation, the urinary bladder transitional epithelium was observed at 1 week after operation. Neogenesis mucosa covered the destroyed area at 2 weeks. The obvious inflammatory cell infiltration and some urinary bladder transitional epithel ium were observed at 1 week in the both two SIS transplanted groups (only SIS and SIS with transinfected cells). At 2 weeks after transplantation, intact epithelium was observed with no significant difference between two groups. But the numbers of neogenesis capillary and positive VEGFR2 expression cells were significantly more in the group of SIS with transinfected cells than those in only SIS group(P ＜ 0.05). At 4 weeks, the number of neogenesis capillary was significantly more than those at 2 weeks (P ＜ 0.05), but there was no significant difference between the only SIS group and SIS with transinfected cells group. Few smooth muscle fibres were observed in both groups at 4 weeks, which at 6 weeks more could be found. At 10 weeks, smooth muscle bundles were observed and there were no significant difference bet ween the two groups of the number of neogenesis capillary and positive VEGFR2 expression cells. CONCLUSION: The role of vascularization induced by VEGF165 transinfected bladder muscular cells in rat tissue engineering bladder with scaffold of small intestinal submucosa(SIS) can be found significantly within 4 weeks. But 6 weeks later, there is no difference between the only SIS group and SIS with transinfected cells group.
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万方数据
维普
