Diversities in expression of neurotensin and calcitonin gene-related peptide in hematological system and gastrointestinal mucosa of rats with acute liver failure
AIM: To discuss the diversities in expression of neurotensin(NT) and calcitonin gene-related peptide (CGRP)in rats with acute liver failure (ALF) and its associativity with hepatic gastrointestinal dysfunction. METHODS: We used thioacetamide (TAA) to induce ALF in rat models and observed their general state of health and liver biochemical index. The expressions of NT and CGRP in the hematological system and gastrointestinal mucosa of ALF rat models were identified by the results of radioimmunoassay and compared with the normal control group. RESULTS: ①The expressions of NT and CGRP in the hematological system and intestinal mucosa and the expressions of NT in the gastric mucosa of the ALF rats were significantly elevated compared with the normal control group (P ＜ 0.01, P ＜ 0.05), whereas the expression of CGRP in gastric mucosa showed no significant change(P ＞ 0.05). ②The linear regression analysis demonstrated a positive correlation between hematic levels of NT and CGRP and levels of ALT and AST (r was 0.56, 0.62, 0.68, 0.70, respectively, P ＜ 0.05). ③With serial NT and CGRP samples at time line 12, 24, 36 and 48 h, the expressions of NT and CGRP increased progressively releventing the degree of the disease, and were significantly higher at 48 h than at 12 h(P ＜ 0.01). CONCLUSION: The expressions of NT and CGRP in hematological system can be used as a marker for liver function and have some significant judgement of the condition of ALF and the prognosis of hepatic encephalopathy and gastrointestinal dysfunction. It is worth to analysis further if that can be a useful model for early diagnosis of hepatic encephalopathy and gastrointestinal dysfunction.
NETL
NSTL
万方数据
维普
