Relationship of multidrug-resistance with AbeM gene in clinical isolates of Acinetobacter baumannii
AIM: To explore the relationship of multidrug-resistance with abeM gene in the clinical isolates of Acinetobacter baumannii. METHODS: Agar dilution method was used to determine the minimum inhibitory concentrations (MICs) of 8 antimicrobials against 37 isolates of Acinetobacter baumannii. AbeM gene and its mRNA expression in Acinetobacter baumannii isolates were detected by PCR and RT-PCR, respectively. RESULTS: The clinical isolates of Acinetobacter baumannii were generally resistant to antimicrobials. Among 37 isolates of Acinetobacter baumannii, 21 strains are resistant (56.76%) and the multidrug-resistant strains account tor 81% in resistant ones. AbeM gene was found in all isolates and the levels of mRNA expression of abeM gene in multidrug-resistant strains were significantly higher compared with the susceptible ones (P ＜ 0.01) and general resistant ones (P ＜ 0.05). CONCLUSION: High level expression of abeM gene was immediately associated with the multidrug-resistance in the clinical isolates of Acinetobacter baumannii.
NETL
NSTL
万方数据
维普
