abeM基因与鲍曼不动杆菌多重耐药性的关系
Relationship of multidrug-resistance with AbeM gene in clinical isolates of Acinetobacter baumannii
王跃 1陈敏2
作者信息
- 1. 成都医学院医学检验系临床微生物学与免疫学教研室,四川,成都,610083
- 2. 湖南省妇幼保健院检验科,湖南,长沙,410008
- 折叠
摘要
目的:探讨abeM基因与临床分离的鲍曼不动杆菌多重耐药性的关系.方法:采用琼脂稀释法检测8种抗菌药物对37株鲍曼不动杆菌的最低抑菌浓度(MICs);以PCR和RT-PCR检测abeM基因的分布及其表达水平.结果:临床分离的鲍曼不动杆菌耐药性高,37株临床分离的鲍曼不动杆菌中,耐药菌株有21株(56.76%),且多重耐药菌株占耐药菌株总数的81%.所有临床分离株均能检测到aheM基因,但多重耐药菌株aheM基因mRNA表达水平显著高于敏感菌株(P<0.01),也明显高于仅对2类或1类抗菌药物耐药的菌株(P<0.05).结论:abeM基因的高表达与临床分离的鲍曼不动杆菌多重耐药性密切相关.
Abstract
AIM: To explore the relationship of multidrug-resistance with abeM gene in the clinical isolates of Acinetobacter baumannii. METHODS: Agar dilution method was used to determine the minimum inhibitory concentrations (MICs) of 8 antimicrobials against 37 isolates of Acinetobacter baumannii. AbeM gene and its mRNA expression in Acinetobacter baumannii isolates were detected by PCR and RT-PCR, respectively. RESULTS: The clinical isolates of Acinetobacter baumannii were generally resistant to antimicrobials. Among 37 isolates of Acinetobacter baumannii, 21 strains are resistant (56.76%) and the multidrug-resistant strains account tor 81% in resistant ones. AbeM gene was found in all isolates and the levels of mRNA expression of abeM gene in multidrug-resistant strains were significantly higher compared with the susceptible ones (P ＜ 0.01) and general resistant ones (P ＜ 0.05). CONCLUSION: High level expression of abeM gene was immediately associated with the multidrug-resistance in the clinical isolates of Acinetobacter baumannii.
关键词
鲍曼不动杆菌/多重耐药性/abeM基因Key words
acinetobacter baumannii/multidrug-resistance/abeM gene引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据