AIM:To elucidate the appropriate dose and time of microwave to induce the apoptosis of UMR 106 osteogenic sarcoma cells. METHODS:Treat the osteogenic sarcoma cells with microwave of different dose and time and use morphology detection, MTT assay, soft agar formation and FCM analysis to examine the apoptosis level. RESULTS: Different dose of microwave caused the morphology change of osteogenic sarcoma cells. With increased dose, more and more cells detached from basal layer. The survival rate of UMR 106 cells decreased a lot following the radiation of microwave with 1 h treatment and demonstrated the dose-dependent trend in MTT assay. The IC50 of microwave radiation on UMR 106 cells was 100 W/m2. FCM analysis showed the G1 arrest of cell cycle in UMR 106 cells under the treatment of microwave radiation. CONCLUSION: Microwave radiation can induce the apoptosis of UMR 106 osteogenic sarcoma cells and the opitimized time and dose were 1 h and 100 W/m2 respectively.
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