Mechanism of RNAi silence inhibiting VEGF on therapy of lung cancer
AIM: To research the mechanism of therapy of cancer with RNAi inhibiting VEGF. METHODS: The pSilencerTM-3.1-VEGF vector was thansfected into the A549 cell line. The mRNA level of Bcl-2 and Bax were detected by RT-PCR. And the expreesion of P53, Bcl-2 and Bax in stable thansfected A549 were analysized by western blot. The apoptosis of stable thasfected A549 was estimated with FCM. RESULTS: The expression of VEGF in the stable thansfected A549 was inhibited obviously. Meanwhile, the P53 , Bcl-2 and Bax expression did not change in the stable transfected AS49 cell lines. And we did not observe the apoptosis of stable transfected A549 cells. Compared with the control group, althrough the Bax expression did not change in the stable transfected A549 cell lines under the HUVEC, the P53 expression increased, while the Bcl-2 decreased, at the sametime, we characterized the apoptosis of transfected A549. What's more, this phenomina could be reversed with the VEGF. CONCLUSION: The mechanism of therapy of cancer with RNAi inhibiting VEGF could result from the blockade the VEGF/VEGFR.
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