胰岛素作用对胰岛β细胞磷脂酶Cγ1表达的影响
Effect of insulin function on expression of phospholipaseCγ1 in β cells
李广文 1焦凯1
作者信息
- 1. 第四军医大学唐都医院内分泌科,陕西,西安,710038
- 折叠
摘要
目的:观察胰岛素对大鼠胰岛β细胞内磷脂酶Cγ1(PLCγ1)表达的影响.方法:大鼠胰岛分离纯化.通过HE染色观察纯化胰岛的形态特征,采用免疫组织化学方法并结合激光扫描共聚焦显微镜和图像分析技术对胰岛β细胞表达不同时间(0,30,60,120,240 min)内的PLCγ1进行半定量分析.结果:PLCγ1在胰腺的内外分泌部均有表达.与0 min比较,胰岛素作用30,60 min时胰岛β细胞表达的PLCγ1荧光强度有明显降低(P<0.05);而在240 min时PLCγ1荧光强度与0min比较无明显差别.结论:胰岛素作用60 min内可使PLCγ1表达下调;胰岛素-PLCγ1途径参与了胰岛素的信息传递.
Abstract
AIM:To observe the effect of insulin on expression of phospholipaseCγ1 in β cells of rat islets. METHODS: Rat islets were isolated and purified. The morphological features were shown with hematoxylin-eosin (HE) dyeing. The expression of PLCγ1 in different times (0, 30, 60, 120 and 240 min) was semiquantitatively analyzed by method of immunocytochemistry combined with laser scanning confocal microscopy and image analyzed technology. RESULTS: PLCγ1 was expressed in the endocrine and exocrine pancreas. The expression of PLCγ1 in β cells for 30 and 60 min with insulin incubation was obviously decreased compared with that at 0 min (P ＜ 0.05). And there was no obvious change at 240 min compared with insulin incubation. CONCLUSION: The expression of PLCγ1 was decreased at 60 min by the inducing of insulin. Insulin - PLCγ1 was concerned with the insulin information transformation.
关键词
磷脂酶Cγ1/胰岛素/胰岛/β细胞Key words
phospholipaseCγ1/insulin/pancreatic islets/β cells引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据