茶多酚表没食子儿茶素-3-没食子酸酯对红细胞膜的促氧化作用
Pro-oxidative effect of epigallocatechin 3-gallate on human erythrocyte membranes
任婧 1张献清 2邢艳菲 3曾成鸣3
作者信息
- 1. 陕西师范大学,化学与材料科学学院,陕西,西安,710062;陕西师范大学,生命科学学院,陕西,西安,710062
- 2. 第四军医大学西京医院输血科,陕西,西安,710033
- 3. 陕西师范大学,化学与材料科学学院,陕西,西安,710062
- 折叠
摘要
目的:研究表没食子儿茶素-3-没食子酸酯(Epigal-locatechin gallate,EGCG)对人红细胞膜的促氧化作用及其分子机制.方法:采用人红细胞膜作为体外实验模型,观察经EGCG处理后细胞形态、膜巯基含量及膜蛋白电泳谱的变化,并与对苯醌的作用进行比较.结果:EGCG可诱导红细胞产生形变和聚集,但这种作用与其促氧化作用无关.在对分离的红细胞膜作用时,EGCG和对苯醌均表现出明显的促氧化作用,降低膜蛋白巯基含量水平,并诱导膜蛋白聚集.结论:EGCG可对红细胞膜产生促氧化作用,这种作用与其在氧化过程中产生的醌式中间体有关.
Abstract
AIM: To explore the pro-oxidative effect of epigallocatechin-3-gallate (EGCG) on erythrocyte membranes and the molecular mechanism of action. METHODS: Human erythrocytes or their membranes were treated with EGCG and p-benzoquinone. Alterations in cellular morphology, glutathione levels and SDS-PAGE patterns of membranes were recorded and compared. RESULTS: The results showed that EGCG induced erythrocyte membrane damages, including changing cellular morphology, inducing sulfhydryl consumption and membrane protein aggregation. The results of p-benzoquinone acting on erythrocyte membranes shared same manner as that of EGCG. CONCLUSION: EGCG played a pro-oxidative effect on erythrocyte membranes through a pathway involved in quinone-like intermediates.
关键词
表没食子儿茶素-3-没食子酸酯/促氧化作用/红细胞膜/对苯醌Key words
epigallocatechin-3-gallate/pro-oxidative/erythrocyte membrane/p-benzoquinone引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据