Effect of ERK1/2 signaling pathway on carbamylated erythropoietin-induced cardio-protection against ischemia-reperfusion injury
AIM: To investigate the effect of extracellular signal-regulated kinase 1/2(ERK1/2) signaling pathway on carbamylated erythropoietin-induced cardioprotection against ischemia-reperfusion (I/R) injury. METHODS: Forty rabbits were randomly allocated to control group, I/R group, I/R + CEPO group, I/R + PD98059 group, I/R + CEPO + PD98059 group. We compared the difference of infarct size using observations of ST ↑, NST↑% , NQ% in the 12-lead ECG. The paraffin slices were subjected to terminal deoxy-nucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining for detection of cardiac myocyte apoptotic rate. RESULTS: There was no diference between I/R + PD98059 group and I/R group on ST↑, NST↑% , NQ% and cardiac myoeyte apoptotic rate (P ＞ 0.05); I/R + CEPO + PD98059 group was higher than I/R + CEPO group on ST↑, NST↑% ,NQ% and cardiac myocyte apoptotic rate (P ＜ 0.05); I/R + CEPO group and IR + PD98059 + CEPO group were lower than I/R group on ST↑, NST↑% , NQ% and cardiac myocyte apoptotic rate (P ＜ 0.05). CONCLUSION: Acute cardioprotective effect of CEPO was mediated by activation of ERK1/2 signaling pathway. ERK1/2 signaling pathway wasn't significantly actived in the absence of CEPO.
NETL
NSTL
万方数据
维普
