多种肿瘤相关抗原联合检测原发性肝癌的诊断价值评价
Evaluation of diagnostic value on primary hepatocellular carcinoma using multiple tumor-associated antigens
任鹏飞 1刘会娟 1闫平平 1代丽萍 2王鹏 2王凯娟 2张建营2
作者信息
- 1. 郑州大学,公共卫生学院流行病学教研室,河南,郑州,450001
- 2. 郑州大学,公共卫生学院流行病学教研室,河南,郑州,450001;郑州大学,蛋白质组学应用研究中心,河南,郑州,450001
- 折叠
摘要
目的:评价14种肿瘤相关抗原(TAAs)联合检测原发性肝癌的诊断价值.方法:利用酶联免疫吸附试验(ELISA)检测115例原发性肝癌患者及153例正常对照血清内的14种TAAs的抗体,利用流行病学筛检试验的评价方法评价TA-As联合检测的诊断价值.结果:单个TAA检测的灵敏度在13.0%～32.2%之间,联合8种TAAs检测其灵敏度增加到72.2%,特异度达到91.2%,阳性似然比为8.49,阴性似然比为0.304,阳性预测值为86.5%,阴性预测值为81.4%,Kappa值为0.65.结论:利用8种TAAs(cyclinE,Koc,IMP1,cIAP,p62,p16,cyclinB1,Calnuc)联合检测原发性肝癌具有较高的诊断价值,为TAAs联合检测作为原发性肝癌早期诊断方法的研究奠定了基础.
Abstract
AIM: To evaluate the diagnostic value of a panel of fourteen tumor-associated antigens (TAAs) on primary hepatocellular carcinoma. METHODS: Autoantibodies against fourteen TAAs in sera from 115 patients with primary hepatocellular carcinoma and 153 normal individuals were detected by enzyme-linked immunosorbent assay (ELISA). Then the diagnostic value was evaluated by epidemiological methods on screening. RESULTS: When each anti-TAA antibody was judged individually, the sensitivity was at a low level from 13.2% to 32.2%, but with the successive addition of TAAs to a final total of eight antigens, there was a stepwise increase of positive antibody reactions reaching a sensitivity of 72.2%, the specificity, positive likelihood ratio, negative likelihood ratio, positive predictive value, negative predictive value and Kappa value were 91.2%, 8.49, 0.304, 86.5%, 81.4% and 0.65, respectively. CONCLUSION: This study demonstrates that a mini-array of eight TAAs including cyclinE, Koc, IMP1, cIAP, p62, p16, cyclinB1 and Calnuc has a high value for diagnosis of primary hepatocellular carcinoma, and that provides an important basis for further research on TAA mini-array as a method in the immunodiagnosis of primary hepatocelluar carcinoma.
关键词
肝细胞癌/肿瘤相关抗原/自身抗体/诊断试验/评价Key words
hepatocellular carcinoma/tumor-associated antigens/auto-antibody/diagnostic test/evaluation引用本文复制引用
基金项目
国家自然科学基金面上项目(30872962)
出版年
2009
NETL
NSTL
维普
万方数据