Evaluation of diagnostic value on primary hepatocellular carcinoma using multiple tumor-associated antigens
AIM: To evaluate the diagnostic value of a panel of fourteen tumor-associated antigens (TAAs) on primary hepatocellular carcinoma. METHODS: Autoantibodies against fourteen TAAs in sera from 115 patients with primary hepatocellular carcinoma and 153 normal individuals were detected by enzyme-linked immunosorbent assay (ELISA). Then the diagnostic value was evaluated by epidemiological methods on screening. RESULTS: When each anti-TAA antibody was judged individually, the sensitivity was at a low level from 13.2% to 32.2%, but with the successive addition of TAAs to a final total of eight antigens, there was a stepwise increase of positive antibody reactions reaching a sensitivity of 72.2%, the specificity, positive likelihood ratio, negative likelihood ratio, positive predictive value, negative predictive value and Kappa value were 91.2%, 8.49, 0.304, 86.5%, 81.4% and 0.65, respectively. CONCLUSION: This study demonstrates that a mini-array of eight TAAs including cyclinE, Koc, IMP1, cIAP, p62, p16, cyclinB1 and Calnuc has a high value for diagnosis of primary hepatocellular carcinoma, and that provides an important basis for further research on TAA mini-array as a method in the immunodiagnosis of primary hepatocelluar carcinoma.
NETL
NSTL
万方数据
维普
