Research of estrogen receptor β gene CA repeated sequence and it's G1082A polymorphism on the coronary artery disease patients
AIM: To study the relationship of estrogen receptorβgene polymorphism and the risk of coronary artery disease. METHODS: ER βCA repeated sequence polymorphism and ERβ gene G1082A polymorphism in 260 male patients with coronary artery diseases and 260 normal male controls was detected using PCR-RFLP method. Lipid profile including triglycerides (TG), total cholesterol (CHO), HDL, LDL and blood sugar level were also detected. RESULTS: The (CA) n ＜ 22 and (CA) n≥22 alleles were designated as S and L,respectively. The frequency of genotypes was as follows: SS(0.248), SL (0.456), LL (0.296) for CA repeat polymorphism, and rr (0.430), Rr (0.420) ,RR(0.150) for G1082A polymorphism. The distribution of genotypes was in Hardy-Weinberg equilibrium. There were significantly different relationships in CAD groups and controls groups in the risk factor of CAD such as hypertension, diabetes, smoking and hypercholesteremia. There were no significantly different relationships in different groups of ER βCA repeated sequence polymorphism and ERβ gene G1082A polymorphism and the other risk factors. There were no relationship between SS and SL genotypes of CA repeated sequence polymorphism and CAD, There were no relationship between rr and Rr of G1082A polymorphism and CAD. But the LL of CA repeated sequence polymorphism and the RR genotype of ERβ gene G1082A polymorphism increased significantly CAD. CONCLUSION: The LL of CA repeated sequence polymorphism and the RR genotype of ERβ gene G1082A polymorphism significantly increased the possibility of CAD other genotypes.
NETL
NSTL
万方数据
维普
