冠心病患者雌激素受体β基因CA重复序列和G1082A多态性研究
Research of estrogen receptor β gene CA repeated sequence and it's G1082A polymorphism on the coronary artery disease patients
杨留才 1钱春野 1成玉生 1林辉2
作者信息
- 1. 盐城卫生职业技术学院,江苏,盐城,224006
- 2. 盐城市第一人民医院,江苏,盐城,224006
- 折叠
摘要
目的:探讨雌激素受体β(Estrogen Receptor Beta,ERβ)CA重复序列和G1082A多态性与冠心病(CAD)的关系.方法:采用PCR-RFLP方法,检测260例男性冠心病患者ERβCA重复序列和G1082A多态性的分布,以260例体检合格者为对照组.结果:对照组中,以CA重复序列平均数22次为界,将重复序列基因分为短基因(<22)和长基因(≥22),分别以S和L表示;CA重复序列基因型SS,SL及LL的频率分别为0.248,0.456和0.296;G1082A基因型rr,Rr及RR的频率分别为0.430,0.420和0.150;基因型频率分布均符合Hardy-Weinberg平衡(P>0.05).在CAD组中,高血压、糖尿病、吸烟、高胆固醇血症等危险因素高于对照组(P<0.01);ERβ基因CA重复序列的SS及SL和G1082A多态性中rr及Rr基因型与CAD的相关主要危险因素均无显著性联系(P>0.05),而CA重复序列的LL基因型和G1082A多态性中RR基因型(突变纯合子)与CAD的发生有联系(P<0.01).但ERβCA重复序列和G1082A多态性与冠心病发生有关,LL和RR基因型人群患冠心病的危险性(OR)显著高于其它基因型的人群.结论:CA重复序列的LL基因型和G1082A的基因型中的突变纯合子(RR)发生冠心病可能性大于其他基因型.
Abstract
AIM: To study the relationship of estrogen receptorβgene polymorphism and the risk of coronary artery disease. METHODS: ER βCA repeated sequence polymorphism and ERβ gene G1082A polymorphism in 260 male patients with coronary artery diseases and 260 normal male controls was detected using PCR-RFLP method. Lipid profile including triglycerides (TG), total cholesterol (CHO), HDL, LDL and blood sugar level were also detected. RESULTS: The (CA) n ＜ 22 and (CA) n≥22 alleles were designated as S and L,respectively. The frequency of genotypes was as follows: SS(0.248), SL (0.456), LL (0.296) for CA repeat polymorphism, and rr (0.430), Rr (0.420) ,RR(0.150) for G1082A polymorphism. The distribution of genotypes was in Hardy-Weinberg equilibrium. There were significantly different relationships in CAD groups and controls groups in the risk factor of CAD such as hypertension, diabetes, smoking and hypercholesteremia. There were no significantly different relationships in different groups of ER βCA repeated sequence polymorphism and ERβ gene G1082A polymorphism and the other risk factors. There were no relationship between SS and SL genotypes of CA repeated sequence polymorphism and CAD, There were no relationship between rr and Rr of G1082A polymorphism and CAD. But the LL of CA repeated sequence polymorphism and the RR genotype of ERβ gene G1082A polymorphism increased significantly CAD. CONCLUSION: The LL of CA repeated sequence polymorphism and the RR genotype of ERβ gene G1082A polymorphism significantly increased the possibility of CAD other genotypes.
关键词
冠心痛/雌激素受体β/CA重复序列多态性/G1082A多态性/危险性Key words
coronary artery diseases/estrogen receptor β/CA repeated sequence polymorphism/G1082A polymorphism/risk引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据