Construction and identification of shRNA eukaryotic expression vectors targeting to prohibitin gene
AIM: To construct 2 shRNA eukaryotic expression vectors targeting to prohibitin gene. METHODS: Finding the sequence of human prohibitin gene in Pubmed, the oligonucleotides of shRNA were designed by the software of Ambion Company then synthesized and directionally cloned into pGenesill. 1 vector. Then the recombinant vectors were extracted and confirmed by restriction enzyme and sequencing analysis. RESULTS: The restriction enzyme analyses demonstrated that shRNA had been inserted into vectors, and sequencing analyses demonstrated that their sequences were the same as the design. CONCLUSION: We have successfully constructed shRNA eukaryotic expression vectors targeting to prohibitin gene. It laid the foundations for the further study on the function of prohibitin gene in tumor cell and the future RNAi experimental research in vivo and in vitro.
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万方数据
维普
