Regulation of Dll4 and Notch1 genetic transcription by HGF in human femoral artery endothelial cells
AIM: To investigate the regulation of D114 and Notch1 genetic transcription mediated by hepatocyte growth factor (HGF) in human femoral artery endothelial cells (HFAECs), and to establish the basis for further investigating the mechanism of HGF promoting arteriogenesis. METHODS: The optimal multiplicity of infection (MOI) was determined by MTT and flow cytometry, after HFAECs were infected with Ad-GFP at the different MOI. Then HFAECs were infected with Ad-HGF at the optimal MOI, HGF in the supernatant was detected by ELISA at 48 h after infecting, meanwhile, the transcription of Notch1 and Dll4 genes was detected by reverse transcription-PCR(RT-PCR). Ad-GFP-infected HFAECs were used as negative controls. RESULTS: Compared to the control group, HGF was obviously detected in the supernatant of Ad-HGF-infected HFAECs,and the up-regulation of Dll4 and Notch1 genes transcription was observed. CONCLUSION: The mechanism of arteriogenesis promoted by HGF might be related with Notch signaling pathway.
NETL
NSTL
万方数据
维普
