HGF对人股动脉内皮细胞中Notch1和Dll4基因转录的调节作用
Regulation of Dll4 and Notch1 genetic transcription by HGF in human femoral artery endothelial cells
高玉芳 1哈小琴 2付晓霞 3吕同德 2唐瑜 2贾庆华2
作者信息
- 1. 兰州大学第二临床医学院,甘肃,兰州,730030;兰州军区兰州总医院实验科,甘肃,兰州,730050
- 2. 兰州军区兰州总医院实验科,甘肃,兰州,730050
- 3. 白银区妇幼保健站,甘肃,白银,730900
- 折叠
摘要
目的:探讨肝细胞生长因子(HGF)对人股动脉内皮细胞(HFAECs)中Notch1和D114基因转录的调节作用,为进一步探讨携带HGF基因的重组腺病毒(Ad-HGF)促动脉血管形成机制奠定基础.方法:用不同感染强度(MOI)的携带绿色荧光蛋白基因的重组腺病毒(Ad-GFP)感染HFAECs后,通过流式细胞仪和MTT法选择最佳MOI,再将Ad-HGF以最佳的MOI感染HFAECs,48 h后收集细胞上清,用ELISA法检测HGF的表达水平,同时提取总RNA,通过反转录-PCR(RTPCR)来检测Notch1和Dll4基因的转录水平.以上均以感染Ad-GFP的细胞作为对照.结果:与对照组比较,Ad-HGF感染细胞后,上清中明显有HGF表达,并且观察到Notch1和Dll4基因转录水平均上调.结论:Ad-HGF促动脉形成机制可能与Notch信号通路有一定的关联性.
Abstract
AIM: To investigate the regulation of D114 and Notch1 genetic transcription mediated by hepatocyte growth factor (HGF) in human femoral artery endothelial cells (HFAECs), and to establish the basis for further investigating the mechanism of HGF promoting arteriogenesis. METHODS: The optimal multiplicity of infection (MOI) was determined by MTT and flow cytometry, after HFAECs were infected with Ad-GFP at the different MOI. Then HFAECs were infected with Ad-HGF at the optimal MOI, HGF in the supernatant was detected by ELISA at 48 h after infecting, meanwhile, the transcription of Notch1 and Dll4 genes was detected by reverse transcription-PCR(RT-PCR). Ad-GFP-infected HFAECs were used as negative controls. RESULTS: Compared to the control group, HGF was obviously detected in the supernatant of Ad-HGF-infected HFAECs,and the up-regulation of Dll4 and Notch1 genes transcription was observed. CONCLUSION: The mechanism of arteriogenesis promoted by HGF might be related with Notch signaling pathway.
关键词
肝细胞生长因子/Notch信号通路/动脉生成/基因转录Key words
hepatocyte growth factor (HGF)/Notch signing pathway/arteriogenesis/genetic transcription引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据