内皮素-1反义脱氧寡核苷酸对糖尿病肾病大鼠肾组织形态学的影响
Protective effects of endothelin-1 mRNA antisense oligodeoxynucleotide on the kidney ultrustructure of streptozotocin-induced diabetic nephropathy rats
余建强 1李玉香 2王洁 3张焱 3王淑静 3袁文俊4
作者信息
- 1. 宁夏医科大学,基础医学院药理学系,宁夏,银川,750004
- 2. 宁夏医科大学,护理学院基础护理学系,宁夏,银川,750004
- 3. 宁夏医科大学,科技中心,宁夏,银川,750004
- 4. 宁夏医科大学,基础医学院生理学系,宁夏,银川,750004;第二军医大学生理学教研室,上海,200003
- 折叠
摘要
目的:研究内皮素-1反义脱氧寡核苷酸(ET-1ASODN)对链脲佐菌素(streptozotoein,STZ)诱导的糖尿病大鼠肾组织形态学的保护作用及其机制.方法:一次性腹腔注射STZ(60 mg/kg),建立大鼠糖尿病模型,观察ET-1AS-ODN预防性给药对大鼠肾组织形态结构的保护作用.结果:ET-1ASODN可明显减轻精尿病大鼠肾肥大指数,改善肾组织形态学损伤.结论:内皮素-1反义寡核苷酸可减轻STZ诱导的糖尿病肾病大鼠的肾损伤,对肾组织具有保护作用.其机制与减少内源性内皮素的产生有关.
Abstract
AIM: To investigate the protective effects of endothelin-1 mRNA antisense oligodeoxynucleotide (ET-1 AS-ODN) on the renal morphology of diabetic nephropathy(DN) and discuss the pathogenesis and mechanism of DN in a diabetic rat model. METHODS: Diabetic rats were induced by intraperitoneal administration of streptozotocin (STZ) at 60 mg/kg. ET-1 AS-ODN was administrated intravenously to the treatment groups and its protective effects on renal morphology were observed. RESULTS: Compared with the DM group, the kidney index reduced significantly the general condition and morphological injury improved significantly in ET-1 AS-ODN treatment groups. CONCLUSION: Endothelin-1 mRNA antisense oligodeoxynucleotide has a protective effect on the kidney by preventing renal impairment in diabetic nephropathy rats. The mechanism of the protective effect of Endothelin-1 mRNA antisense oligodeoxynucleotide involves inhibition of endogenous ET-1 production.
关键词
内皮素-1反义寡核苷酸/糖尿病肾病/形态学/内皮素Key words
endothelin-1 mRNA antisense oligodeoxynucleotide/diabetic nephropathy/morphology/endothelin引用本文复制引用
基金项目
宁夏高等学校科学技术研究项目(宁教高[2007]292号-103)
宁夏高等学校科学技术研究项目(宁教高[2007]292-87)
宁夏卫生厅重点科研计划(W2008087)
宁夏医科大学科学技术研究项目(XM200832)
出版年
2009
NETL
NSTL
维普
万方数据