阿魏酸钠对低氧性肺动脉高压大鼠肺血管平滑肌细胞Caspase-3,Bcl-2表达的影响
Effect of sodium ferulate on Caspase-3 and Bcl-2 expression levels of smooth muscle cells in pulmonary arterv of hypoxic pulmonary hypertensive rats
陈国柱 1程德云 2聂英2
作者信息
- 1. 第三军医大学新桥医院急诊科,重庆,410037
- 2. 四川大学华西医院呼吸内科,四川,成都,610041
- 折叠
摘要
目的:探讨阿魏酸钠(SF)对低氧性肺动脉高压大鼠肺小动脉平滑肌细胞Caspase-3,Bcl-2表达的影响.方法:将21只Wistar大鼠随机分为对照组(n=7)、低氧组(n=7)及干预组(n=7)(胃饲SF并进行低氧处理).以常压低氧复制肺动脉高压模型,右心导管插入法检测平均肺动脉压(mPAP);分别称量大鼠右心室(RV)和左心室加室间隔(LV+S)的质量,计算出右心室肥厚指数RV/(LV+S);图像分析法测量肺小动脉管壁厚度和面积,计算出肺小动脉管壁厚度指标即管壁厚度占血管外径的百分比(WT%)和管壁面积占血管总面积的百分比(WA%);进行Caspase-3,bcl-2免疫组化染色,比较各组Caspase-3和bcl-2积分光密度值(IOD).结果:①与对照组比较,低氧组大鼠平均肺动脉压(mPAP)明显升高(P<0.05)、肺小动脉WT%,WA%以及RV/(LV+S)明显增加(P<0.05).与低氧组比较,干预组大鼠平均肺动脉压(mPAP)明显降低(P<0.05)、肺小动脉WT%,WA%以及RV/(LV+S)明显减少(P<0.05).②与对照组和低氧组分别比较,干预组Caspase-3 IOD值均显著增高(P<0.05),且与对照组和低氧组分别相比,十预组bcl-2表达均处于弱势,IOD值均显著降低(P<0.05).与对照组相比,低氧组无统计学差异(P>0.05).结论:SF可造成低氧性肺动脉高压肺血管平滑肌细胞Caspase-3表达上调及Bcl-2表达下调.
Abstract
AIM: To investigate the impact of sodium femlate on Caspase-3 and Bcl-2 of smooth muscle cells in pulmonary arterioles of hypoxic pulmonary hypertensive rats. METHODS: Twenty-one male Wistar rats were randomly divided into control group(n = 7), hypoxic group(n =7) and sodium ferulate treated group(n = 7). Mean pulmonary arterial pressure (mPAP) was measured by a right cardiac catheterization. Dry weight of right ventricle(RV), left ventricle with septum(LV + S) were determinded and RV/( LV + S) ratio was calculated. The index of wall thickness of pulmonary arterioles which are the percentage of vascular wall thickness/vascular external diameter (WT%) and the percentage of vascular wall area/total vascular area (WA%) was measured by a computerized image analyzer. The distribution of Caspase-3 and Bcl-2 in smooth muscle cells of pulmonary aterioles were observed by streptavidin peroxidase method (SP), the morphologic changes of small pulmonary arteries and the integrated optical density (IOD) of Caspase-3 and bcl-2 were determined by image analysis technique. The comparisons of the IOD in VSMC in pulmonary vessels among three groups were made to assess the remodeling of small pulmonary arteries. RESULTS: ①In comparison with the control group, the mPAP of rats had been obviously elevated after hypoxia (P ＜ 0.05), as well as WT%, WA% of pulmonary arterioles and RV/(LV + S) (P ＜ 0.05). The mPAP, WT%, WA% of pulmonary arterioles and RV/(LV + S) were much lower in rats of hypoxia with sodium ferulate group than those of hypoxia group (P ＜ 0.05). ②The expression level of Caspase-3 in hypoxia group was lower than that in hypoxia + sodium ferulate group (P ＜ 0.05), the expression level of bcl-2 in hypoxia group was higher than that in hypoxia + sodium ferulate group (P ＜ 0.05). There were no significant difference between the control group and the hypoxia group (P ＞ 0.05). CONCLUSION: Sodium ferulate can increase Caspase-3 and decrease bcl-2 of PASMC.
关键词
低氧血症/肺动脉高压/阿魏酸钠/Caspase-3/Bel-2Key words
hypoxia/pulmonary hypertension/sodium ferulate/Caspase-3/Bcl-2引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据