首页|高压氧对缺氧缺血性脑损伤新生大鼠caspase-3及caspase-9表达的影响

高压氧对缺氧缺血性脑损伤新生大鼠caspase-3及caspase-9表达的影响

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目的:通过检测caspase-3,caspase-9的表达情况,探讨高压氧(HBO)对缺氧缺血新生大鼠脑细胞凋亡的影响及分子机制.方法:建立缺氧缺血性脑损伤(HIBD)大鼠模型,将其分为HIBD组(24只)及HBO干预组(24只),同时设立假手术(Sham)组(24只).分别于造模后18,24,48和96 h4个时间点断头取脑观察其病理改变(海马及皮层区),并用免疫组化的方法检测caspase-3及caspase-9的蛋白表达.结果:HIBD组18,24,48和96 h时间点海马及皮层区caspase-3,caspase-9蛋白的表达均明显高于Sham组(P<0.05),HBO十预组则显著缓解,caspase-3,caspase-9表达的升高程度均低于HIBD组(P<0.05).结论:HBO能减轻新生大鼠HIBD模型海马及皮层区脑细胞的凋亡,其作用可能是通过下调caspase-9进而影响caspase-3的活性表达来完成的.
Effect of hyperbaric oxygen-treatment on the expression of caspase-3 and caspase-9 in the newborn rats brain of hypoxic-ischemia
AIM: To explore the effect of Hypcrbaric Oxygen (HBO) on the apoptosis resulted from Hypoxic-Ischemic Brain Damage(HIBD), and to observe the change of Caspase-3 and Caspase-9 expression in this process. METHODS: The hypoxia-ischemia models were divided into following 3 groups: HIBD model group (24 cases), HBO treatment group (24 cases) and sham operation group(24 cases). The brain were taken from the model at 18, 24, 48 and 96 h after the model was made by decapitation. Then observe the morphologic change of brain tissues (the hippocampus and the cortex) and check the dynastic change of Caspase-3 and Caspase-9 expression by immunohistochemistry at different time points. RESULTS: The expression of Caspase-3 and Caspase-9 in the hippocampus and cortex was higher than that of sham group at 18, 24, 48 and 96 h time point. Compared with HIBD(P < 0.05). The increased rate of Caspase-3 and Caspase-9 expression was significantly decreased at each time point by HBO treatment(P < 0.05). CONCLUSION: HBO decreased the apoptosis of hippocampus and cortex nerve cells on 7-day-old HIBD rat model. It is suggested that through down-regulation of Caspase-9 and decrease of the expression of Caspase-3, HBO treatment prevent brain cells from apoptosis.

caspase-3caspase-9apoptosishyperbaric oxygenhypoxic-ischemic brain damage

牛彦平、王玲、檀国军、姜建勇

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河北医科大学,第二医院康复科,河北,石家庄,050000

河北医科大学,第三医院神经内科,河北,石家庄,050000

河北医科大学,第二医院神经内科,河北,石家庄,050000

caspase-3 caspase-9 凋亡 高压氧 缺氧缺血性脑损伤

河北省科学技术委员会科技攻关计划

052761527

2009

第四军医大学学报
第四军医大学

第四军医大学学报

CSTPCDCSCD北大核心
影响因子:0.599
ISSN:1000-2790
年,卷(期):2009.30(20)
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