Effect of IGF-I on secretory function of mouse T,B lymphocytes
AIM: To explore the possibility of the IGF-1 treatment on immune dysfunction in stressed situation, the insulin-like growth factor-1 (IGF-1) on the effect of T, B cell secretory function was observed. METHODS: Using enzymelinked immunosorbent assay(ELISA) analyzed the effect of IGF-1 on mouse T cell secretion of interleukin-2 (IL-2) and B cells secretion of immunoglobulin(Ig) in immune response to antigen in vitro. RESULTS: Mice T, B cells administrated 80 g/L IGF-1 were cultured for 3 d, the IL-2 activity in culture supernatant increased 366.74 nkat/mL as compared with the control group (P ＜ 0.01), and that were cultured for 6 d the IgG content in normal mice T, B cells culture supernatant and stressed mice T, B cells culture supernatant were higher separately than that control group. Especially the IgG content in stressed mice T, B cells culture supernatant increased 41 μg/L as compared with the control group(P ＜ 0.01). CONCLUSION: In the presence of IGF-1 the mouse T, B cell stimulated by antigen cultured in vitro, the ability of T cells secreted IL-2 and B cell secreted IgG were enhanced. It is suggest that IGF-1 may have a modulatory effect on the immune response.
insulin-like growth factor 1T, B lymphocytesimmunomodulation
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NSTL
万方数据
维普
