女贞子总黄酮对高脂模型大鼠脂代谢的影响
Lipometabolism effect of total flavonoid in ligustrum lucidum ait on hyperlipidemic model
曹兰秀 1周永学 1顿宝生 1赵娴1
作者信息
- 1. 陕西中医学院方剂教研室,陕西,咸阳,712046
- 折叠
摘要
目的:研究女贞子总黄酮对高脂模型大鼠脂代谢的影响并探讨其作用机制.方法:以Triton诱导大鼠高脂模型,女贞子总黄酮以15,45,150 me/kg体质量给药3 d,以苯扎贝特为阳性对照,生化方法检测总胆固醇(TC),三酰甘油(TG)水平,逆转录-多聚酶链反应(RT-PCR)检测肝组织脂蛋白脂酶(LPL)、过氧化物酶体增殖物激活受体α(PPARα)及羟甲戊二酰辅酶A还原酶(HMGCR)mRNA的表达水平.结果:模型组TC,TG水平显著增高,PPARα,LPL表达明显降低,而HMGCR表达增强.女贞子总黄酮可显著降低TC,TG水平;促进PPARα,LPL的表达,抑制HMGCR的表达.结论:女贞子总黄酮对高脂模型大鼠脂代谢紊乱具有较好的调节作用,可能通过对PPARα-LPL通路以及HMGCR表达的调控来实现其降脂作用.
Abstract
AIM: To explore the effect of total flavonoid in ligustrum lucidum ait(TFL) on lipid metabolite of high-lipid rats, and to clarify the related mechanism. METHODS: The hyperlipidemic model was induced by abdominal injection of Triton WR-1339. Benzafibrate was used as positive control. The hyperlipidemic rat were treated with TFL with doses of 15, 45 and 150 mg/kg for 3 d, respectively. The contents of triglyceride (TG) and total cholesterol (TC) of the model were detected by biochemical method. The expression level of lipoprotein (LPL), 3-hydroxy-3-methylglutary-coenzyme A reductase (HMGCR) and peroxisome prolifemiceor-actived receptors α(PPARα) mRNA in liver mucosa were determined by reversed transcription polymerase chain reaction (RT-PCR) method. RESULTS: The model developed hyperlipidemia which TG and TC were elevated significantly; the expression level of LPL and PPARα of hyperlipidemic rat were significantly decreased; the expression level of HMG-CoA reductase significantly increased. Treatment of the model with different dose of TFL significantly decreased TC and TG level in blood and promoted the expression level of LPL and PPARα, and suppressed the expression level of HMGCR. CONCLUSION: TFL has the activity of modulating lipid metabolite of high-lipid rat. The potential mechanisms of TFL's hypolipidmic effects may be regulating lipoprotein degradation through PPARα-LPL pathway, or regulating steroid biosynthesis by reducing HMGCR.
关键词
女贞子总黄酮/脂代谢/高脂模型/肝组织脂蛋白脂酶/过氧化物酶体增殖物激活受体仅/羟甲戊二酰辅酶A还原酶Key words
total flavonoid in ligustrum lucidum ail/lipometabolism/hyperlipisdemia model/lipopprotein/3-hydroxy-3-methylglutary-coenzyme A redutasein/peroxisome proliferator-actived receptors α引用本文复制引用
基金项目
陕西省教育厅自然科学基金(07JK218)
出版年
2009
NETL
NSTL
维普
万方数据