Expression of TLR-4,TNF-α and NF-κB in rat cardial muscles during ischemia-reperfusion
AIM: To investigate the expression of TLR-4, TNF-α and NF-κB in rat cardial muscles during ischemia reperfusion. METHODS: Twenty-fire female SD rats were randomly divided into sham operation group (n = 5), ischemia group (n = 5), ischemia reperfiision 30 min group (n = 5), ischemia reperfusion 60 min group (n = 5), ischemia reperfusion 90 min group (n = 5), Expression level of TLR4, TNF-α were measured by RT-PCR. RESULTS: The expression level of TLR4, TNF-α and NF-κB significantly increased after ischemia-reperfusion, there are positive correlation among them. CONCLUSION: These data suggest that myocardial ischemia-reperfusion can result in intensive activation of TLR4, TNF-α and NF-κB. Treatment with inhibitors to TLR4 may be benefit for attenuation of myocardial injury induced by ischemia- reperfusion and subsequent acute inflammatory response.
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万方数据
维普
