Effect and mechanism of kallikrein from agkistrodon acutus venom to the cerebral ischemia-reperfusion rats
AIM: To explore the effect and the mechanism of kallikrein from Agkistrodon acutus venom to rats of cerebral ischemic-reperfusion. METHODS: All rats were randomly divided into sham operation group, control group of physiological saline and treatment group. The treatment group rats were intraperitoneally administrated with kallikrein at dose of 17.5 U/(kg · d) for 3 d. After 3 d, neurological function was appraised; infarct volume was determined, pathology and Nissl's staining in ischemic tissue were detected. And the immunohistochemistry of NSE, GFAP and vWF were detected. RESULTS: The delayed application kallikrein from Agkistrodon acutus venom to rats of cerebral ischemic-reperfusion can obviously reduce the loss of neurological function and the infarct volume. It can also relieve the change of pathology and improve the Nissl's staining in the ischemic tissue. It can obviously increase the positive cells of NSE, GFAP and vWF immunohistochemistry. There were obvious statistics difference (P ＜ 0.05) between group B and group C. CONCLUSION: Delayed application kallikrein from Agkistrodon acutus venom to rats of cerebral ischemic-reperfusion has protective effect to cerebral ischemia. The protective mechanisms were advancing neurogenesis, neurogliocyte regeneration and angiogenesis in the ischemic region.
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万方数据
维普
