IGF-1对精原细胞MAPK途径信号转导的影响
Effect of IGF-1 on the signal transduction pathway of the MAPK route in the spermatogenous cell
沈国球 1鲁功成 2潘铁军 1肖亚军2
作者信息
- 1. 广州军区武汉总医院泌尿外科,湖北,武汉,430070
- 2. 华中科技大学同济医学院协和医院泌尿外科,湖北,武汉,430000
- 折叠
摘要
目的:研究胰岛素样生长因子1(IGF-1)对大鼠精原细胞MAPK途径分子Erk1和2磷酸化的影响作用.方法:分离并培养大鼠精原细胞,采用不同浓度的IGF-1刺激培养的精原细胞.采用Western Blot方法检测Erk1/2分子的磷酸化.结果:成功获得体外培养的精原细胞,IGF-1刺激后精原细胞Erk1/2分子的磷酸化水平显著增强.IGF-1浓度与Erk1/2磷酸化水平呈正相关关系.结论:IGF-1增强Erk1/2分子的磷酸化水平可能是IGF-1促进精原细胞增殖和提高大鼠生精能力的重要分子机制.
Abstract
AIM: To investigate the influence of cytokine IGF-1 on the phosphorylation situation of MAPK pathway molecular Erk1 and Erk2 in spermatogenous cell. METHODS: Separate and transiently culture the rat spermatogenous cell, the phosphorylation of Erk1/2 was detected with Western Blot in spermatogenous cell treated with serials concentrations of IGF-1. RESULTS: The spermatogenous cells were obtained successfully and transciently cultured for more than 12 h. Cytokine IGF-1 could enhance the phosphorylation level of MAPK pathway molecular Erk1/2 in various degree. The higher the concentration of IGF-1 is, the more intensive the phosphorylation of Erk1/2 is. CONCLUSION: Cytokine IGF-1 can promote the proliferation of spermatogenous cells by elevating the phosphorylation level of Erk1/2. These suggest the IGF-1 is an important factor to support the spermatogenic capability.
关键词
胰岛素样生长因子-1/磷酸化/精原细胞Key words
IGF-1/phosphorylation/spermatogenous cells引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据