Effect of IGF-1 on the signal transduction pathway of the MAPK route in the spermatogenous cell
AIM: To investigate the influence of cytokine IGF-1 on the phosphorylation situation of MAPK pathway molecular Erk1 and Erk2 in spermatogenous cell. METHODS: Separate and transiently culture the rat spermatogenous cell, the phosphorylation of Erk1/2 was detected with Western Blot in spermatogenous cell treated with serials concentrations of IGF-1. RESULTS: The spermatogenous cells were obtained successfully and transciently cultured for more than 12 h. Cytokine IGF-1 could enhance the phosphorylation level of MAPK pathway molecular Erk1/2 in various degree. The higher the concentration of IGF-1 is, the more intensive the phosphorylation of Erk1/2 is. CONCLUSION: Cytokine IGF-1 can promote the proliferation of spermatogenous cells by elevating the phosphorylation level of Erk1/2. These suggest the IGF-1 is an important factor to support the spermatogenic capability.
NETL
NSTL
万方数据
维普
