Preparation of SD rat animal model of bladder tumor and its testing of HER-2
AIM: To establish an animal model of bladder cancer in SD rats by perfusing N-methyl-N-nitrosourea into bladder, and test its expression of HER-2. METHODS: Fifty SD rats were randomly divided into experimental group and control group, the experimental group perfused with MNU(2.5 mg everytime, one time every 3 weeks, 4 times in all) into bladder, the control group with physiological saline solution. The bladders were slited and the biopsies were gotten respectively at the 11th week. Immunohistochemical SP method was applied to detect HER-2 protein of tumor tissues and RT-PCR method was applied to detect HER-2 mRNA. RESULTS: There wasn't any tumor in control group, but the experimental group showed bladder cancers in 95% SD rats. Its morphology and histological features were similar to human bladder cancer. Immunohistochemistry and RT-PCR showed parts of HER-2 protein and HER-2 mRNA were positive. CONCLUSION: We successfully established a HER-2-positive animal model of bladder tumor perfusing bladder with MNU.
NETL
NSTL
万方数据
维普
