Electrospinning nanofiber of β-tricalcium phosphate/gelatin biomimetics for graded tissue regeneration membrane
AIM: To prepare a kind of gelatin (Gel) with β-tricalcium phosphate (β-TCP) hybrid nanofibrous membrane and to test its biocompatibility for guided tissue regeneration. METHODS: The hybrid nanofibrous membrane is made by electrospinning technique. Through the ways of scanning electron microscopy(SEM), mechanical tensile test, MTT test and cell co-culture with the material to character the material and study its biocompatibility. RESULTS: After crossing-linking the average diameter of hybrid nanofibrous were increased from 200-300 nm to 500-600 nm. By cross-linking its tensile fracture stress is markedly improved from (1.08 ± 0.24) MPa to (6.47 ± 0.85) MPa. In regard to cytotoxicity there is significant difference between the positive control and the hybrid nanofibrous membrane (P ＜ 0.01), and there is no significant difference compared with the negative control. At last, the osteoblast-like cells (MG-63) could successfully attach and proliferate on the hybrid nanofibrous membrane. CONCLUSION: The B-tricalcium phosphate/gelatin guided tissue regeneration membrane can be prepared by the electrospinning technique successfully. Because of its good biocompatibility , the hybrid nanofibrous membrane can be used for tissue engineering, indicating that the hybrid nanofibrous membrane is a promising material for periodontal tissue regeneration.
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万方数据
维普
