电纺β-磷酸三钙/明胶引导组织再生膜的制备及研究
Electrospinning nanofiber of β-tricalcium phosphate/gelatin biomimetics for graded tissue regeneration membrane
郑钧元 1唐明 1邓旭亮 2杨小平 3张慎 3李晓霞1
作者信息
- 1. 重庆医科大学附属第一医院口腔科,重庆,400016
- 2. 北京大学口腔医学院,北京,100081
- 3. 北京化工大学北京市新型高分子材料制备与加工重点实验室,北京,100029
- 折叠
摘要
目的:制备一种β-磷酸三钙(β-TCP)与明胶(Gel)杂化的纳米纤维引导组织再生膜,并对其生物相容性进行研究,为新型牙周组织再生材料的研制提供理论依据.方法:采用静电纺丝技术制备β-磷酸三钙与明胶的杂化纤维膜.通过扫描电子显微镜(SEM)和力学拉伸实验对杂化纤维膜进行表征,通过MTT实验及细胞与材料共培养对其生物相容性进行评价.结果:杂化纤维膜交联前、后其纤维的平均直径分别为200～300 mm和400～500 mm,在交联之后其拉伸断裂应力从(1.08±0.24)MPa提高到(6.47±O.85)MPa.杂化纤维膜在细胞毒性上与阳性对照间有统计学差异(P<0.01),与阴性对照无统计学差异,并且人成骨样细胞(MG-63)可以成功的贴附生长在该材料上.结论:β-磷酸三钙/明胶纳米纤维引导组织再生膜可以成功地用电纺丝法制备出来,且其生物相容性较好,表明该材料町以用于组织工程学的研究,是一个具有很好应用前景的牙周组织工程再生材料.
Abstract
AIM: To prepare a kind of gelatin (Gel) with β-tricalcium phosphate (β-TCP) hybrid nanofibrous membrane and to test its biocompatibility for guided tissue regeneration. METHODS: The hybrid nanofibrous membrane is made by electrospinning technique. Through the ways of scanning electron microscopy(SEM), mechanical tensile test, MTT test and cell co-culture with the material to character the material and study its biocompatibility. RESULTS: After crossing-linking the average diameter of hybrid nanofibrous were increased from 200-300 nm to 500-600 nm. By cross-linking its tensile fracture stress is markedly improved from (1.08 ± 0.24) MPa to (6.47 ± 0.85) MPa. In regard to cytotoxicity there is significant difference between the positive control and the hybrid nanofibrous membrane (P ＜ 0.01), and there is no significant difference compared with the negative control. At last, the osteoblast-like cells (MG-63) could successfully attach and proliferate on the hybrid nanofibrous membrane. CONCLUSION: The B-tricalcium phosphate/gelatin guided tissue regeneration membrane can be prepared by the electrospinning technique successfully. Because of its good biocompatibility , the hybrid nanofibrous membrane can be used for tissue engineering, indicating that the hybrid nanofibrous membrane is a promising material for periodontal tissue regeneration.
关键词
明胶/β-磷酸三钙/引导组织再生/静电纺丝/生物相容性Key words
gelatin/β-tricalcium phosphate/guided tissue regeneration/electrospinning/biocompatibility引用本文复制引用
基金项目
国家高技术研究发展计划(863计划)目标导向项目(2007AA03Z328766)
出版年
2009
NETL
NSTL
维普
万方数据