Nanometer hydroxyl apatite root canal backfill material basic research
AIM: Through (backfill disperses formaldehyde cresol compound preparation to the nanometer hydroxyl apatite root sufficient material and the traditional root canal backfill material with compound prescription muskdeer vanilla phenol) to conduct the cell toxicity, the root canal are closed the contrast research. Appraisal nanometer hydroxyl apatite material cell toxicity, root canal wall being closed. METHODS: ①Cell toxicity experiment: In vitro raise osteoblast, the increase nanometer hydroxyl apatite material leaching solution, and take the nutrient fluid as the negative control group, fills disperses the formaldehyde cresol compound cataplasma with the compound prescription muskdeer vanilla phenol for the masculine control group. Uses the MTT law according to the ISO standard to carry on in vitro cytotoxin experiment, the test soaks differently proposes the time the material leaching solution and the osteoblast contacts 1, 3, 5, 7 d the A values, appraises his/her the cell toxicity. ②The root canal is closed the experiment: Divide into 2 groups stochastically 10 new wiping out single rooted teeth, each group of toeet carry on the root canal preparation according to the conventional method. The experimental group is 400 g/L nanometer hydroxyl apatite cataplasma backfill, the control group disperses the formaldehyde cresol compound cataplasma backfill for the backfill with the compound prescription muskdeer vanilla phenol. Makes X piece to guarantee in each group tests the exsomatize tooth root of a tooth to consummate fills, after hardens fully, carries on the micro leakage experiment. The improvement glucose examines the micro leakage analysis installment to carry on the root canal being closed experiment , after 30 d the result determines by the glucose quota analyzer. RESULTS: ① Cell toxicity experiment: The experimental group and the negative control group non-statistics difference, the experimental group and the masculine control group have statistics difference(P ＜ 0.05). ②The root canal is closed the experiment : The control group glucose density is higher than the experimental group obviously, has statistics difference (P ＜ 0.05). CONCLUSION: The nanometer hydroxyl apatite root sufficient material in vitro cell toxicity experiment negative, has the good biological compatibility. Nanometer hydroxyl apatite root sufficient material it surpasses the backfill with the root canal wall being closed aspect to disperse the formaldehyde cresol compound cataplasma obviously with the compound prescription muskdeer vanilla phenol.
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