Synthesis and determination of pig chondroitin sulfate
AIM: To improve the preparing method of pig suliuric acid chondroitin. METHODS: The thin alkali-thick salt method was modified to prepare pig sulfuric acid chondroitin. And then the infrared spectrometry and the carbazole spectrophotometric method were used to measure the quantity and structure of pig sulfuric acid chondroitin. RESULTS: The homemade pig sulfuric acid chondroitin and the commercial pure sulfuric acid chondroitin were compared using infrared absorption spectrum. It showed that both of them had the peak of absorption at 3400, 1620, 1560, 1240 and 860 cm-1. The mass chroma of homemade pig sulfuric acid chondroitin was in range of 0.00-0.25 g/L. The correlation coefficient r was 0.9998(n = 7). The returns-ratio was 99.48% and RSD was 0.73%. CONCLUSION: The modified method is much simple and fast. The result is accurate. It is indicated that it could be used as the one of the preparation methods of pig sulfuric acid chondroitin.
Sulfuric acid chondroitinthin alkali-thick salt methodinfrared spectrometrycarbazole method
NETL
NSTL
万方数据
维普
