TPX2在食管鳞癌中mRNA的表达及与临床病理的关系
mRNA expression of TPX2 and its reference with clinical pathology in esophageal cancer
刘红春 1刘玉含 2刘红彦 3朱长连4
作者信息
- 1. 郑州大学,第一附属医院检验科,河南,郑州,450052;郑州大学,基础医学院病理学与病理生理学,河南,郑州,450052
- 2. 郑州大学,第一附属医院检验科,河南,郑州,450052
- 3. 郑州大学,基础医学院病理学与病理生理学,河南,郑州,450052
- 4. 郑州大学,基础医学院病理学与病理生理学,河南,郑州,450052;郑州大学,第三附属医院儿科,河南,郑州,450052
- 折叠
摘要
目的:检测TPX2在食管癌组织中的表达状态,探讨TPX2与食管癌临床及病理参数的关系.方法:运用RTPCR方法检测62例食管鳞癌组织及其对应的癌前病变组织和正常食管黏膜组织TPX2的mRNA表达水平;探讨TPX2与食管癌临床及病理参数的关系.结果:TPX2在正常食管上皮中不表达或者弱表达,食管癌组织中TPX2的表达率为65.5%(62/40),食管癌旁上皮组织为35.5%(22/62),TPX2在食管癌组织中的表达显著高于食管癌旁上皮及正常上皮组织.且表达水平与食管癌的淋巴结转移和浸润深度相关,组间比较差异有统计学意义(P<0.01).而在不同分级、性别和年龄患者中,TPX2的表达差异无统计学意义.结论:TPX2的表达与食管癌的浸润深度及转移有一定的相关性.
Abstract
AIM: To explore the expression in esophageal squamous cell cancer (ESCC) and approach relationship between the TPX2 and clinicopathological characteristic of esophageal squamous cell carcinoma. METHODS: The expression of TPX2 in 62 specimens of ESCC, paracancer esophageal, epithelia and distant normal esophageal epithelia was detected by reverse transcription-polymerase chain reaction (RT-PCR). The correlations of TPX2 expression to clinicopathologic features of the 62 ESCC patients were analyzed. RESULTS: The positive rate of TPX2 was significantly higher in ESCC than in paracancer esophageal epithelia (65.5% vs 35.5%); The expression of TPX2 was correlated with the histological grade, infiltrative depth and lymphatic metastasis in esophageal squamous cell carcinoma. There was a significant statistic difference among the groups (P ＜ 0.01). The expression of TPX2 had no correlations to grade, sex, and age. CONCLUSION: The expression of TPX2 might be related to the infiltrative depth and lymphatic metastasis in esophageal squamous cell carcinoma.
关键词
食管鳞癌/TPX2/RT-PCR检测/临床病理学Key words
esophageal neoplasm/TPX2/RT-PCR/clinical pathology引用本文复制引用
基金项目
河南省教育厅自然科学基金(2009A310011)
出版年
2009
NETL
NSTL
维普
万方数据