首页|不同类型冠心病患者外周血树突状细胞分型及功能研究

不同类型冠心病患者外周血树突状细胞分型及功能研究

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目的:研究不同类型冠心病患者外周循环中树突状细胞(DCs)数量和功能的变化,探讨DCs与冠脉粥样硬化斑块从稳定到"易损"的关系.方法:80入选者被分为稳定型心绞痛组(SAP)(17例)、肌钙蛋白阴性不稳定型心绞痛组[UAP(CTNI-),15例]、肌钙蛋白阳性不稳定心绞痛组[UAP(CTNI+),15例]、急性心肌梗死组(AMI)(15例),冠状动脉造影阴性对照组(CTL)(18例).流式细胞4色分析法检测DCs及亚型的比例;流式细胞仪及酶联免疫吸附法(ELISA)检查DCs功能.结果:与CTL组相比,SAP患者外周血DCs占外周血白细胞的比例和数量无显著变化;UAP(CTNI-)患者DCs比例和数量显著增高;AMI患者DCs比例和数量均减少;UAP(CTNI+)患者DCs比例降低.培养第7日,UAP(CTNI-),UAP(CTNI+),AMI组CD83表达高于CTL,SAP组,UAP(CTNI+)、AMI组显著高于UAP(CTNI-)组(P<0.01).培养第7日,在DCs数量为2×10~8/L时,UAP(CTNI-),UAP(CTNI+),AMI组DCs刺激T细胞增殖的能力显著高于CTL,SAP组(P<0.01);UAP(CTNI+),AMI组显著高于UAP(CTNI-)组(P<0.01).混合淋巴细胞反应上清液中IL-12,IL-10,IFN-α浓度:UAP(CTNI-),UAP(CTNI+),AMI显著高于CTL,SAP组;UAP(CTNI+)、AMI组显著高于UAP(CTNI-)组(P<0.01),AMI组较UAP(CTNI+)组有升高趋势.结论:检测冠心病外周血DCs数量、比例及功能可以一定程度反应冠心病的进展.
Classification and functional study of peripheral blood dendritic cells in different types of coronary artery disease
AIM: To study the changes of the percentage and absolute numbers in peripheral blood dendritic cells(DCs) within the leukocyte population and their functions in different types of coronary artery disease (CAD) so as to explore the relation between the DCs and the development of CAD. METHODS: Sixty-two patients diagnosed as CAD initially were divided into 4 groups: 15 patients in AMI group, 15 patients inUAP(CTNI +) groups, 15 patients in UAP(CTNI -) group and 17 patient in SAP group. Eighteen patients in the control group with negative result in coronary angiography The percentage of peripheral blood DCs Subsets were measured by four color flow cytometry. The functions of DCs were measures by ELISA and flow cytometry. RESULTS: The percentage and numbers of DCs within the leukocyte population was similar for normal in SAP, while significantly higher in the UAP (CTNI -), lower in AMI when compared the control group, and there was no changes of the numbers in UAP(CTNI +). We found that the CD83 were higher in DCs of UAP (CTNI -), UAP (CTNI +), AMI groups after cultured the DCs 7 d in vitro. T-cell proliferation in UAP(CTNI -), UAP(CTNI +) and AMI incubated with 2 × 108/L DCs evoked a strong proliferation, which compared with CTL and SAP (P ＜ 0.05, respectively). The IL-12, IL-10 and IFN-α in supernatant of mixed lymphocyte reaction were higher in UAP (CTNI -), UAP (CTNI +) and AMI. than CTL. CONCLUSION: The changes of the percentage and absolute numbers in peripheral blood DCs within the leukocyte population and their functions in different types of CAD can predict the progression of coronary artery plaques.

dendritic cellcoronary artery diseaseatherosclerotic plaqueimmune function

涂晓文、李志梁、刘映峰、魏向龙、赵欢

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南方医科大学珠江医院心内科,广东,广州,510282

树突状细胞 冠心痛 粥样斑块 免疫功能

南京军区重点课题

08Z016

2009

第四军医大学学报
第四军医大学

第四军医大学学报

CSTPCDCSCD北大核心
影响因子：0.599
ISSN：1000-2790
年,卷(期)：2009.30(20)
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