丰富环境对快速老化小鼠SAMP8情感的影响
Effect of enriched environment on emotional behaviour in senescence accelerated-prone mice 8
苑振云 1顾平 2刘力 3崔冬生 3刘静 3王彦永 2耿媛 3朱爱萍 3马琳 3王铭维2
作者信息
- 1. 河北医科大学第一医院,急诊科,河北,石家庄,050031;河北医科大学第一医院,河北省脑老化与认知神经科学实验室,河北,石家庄,050031
- 2. 河北医科大学第一医院,河北省脑老化与认知神经科学实验室,河北,石家庄,050031;河北医科大学第一医院,神经内科,河北,石家庄,050031
- 3. 河北医科大学第一医院,河北省脑老化与认知神经科学实验室,河北,石家庄,050031
- 折叠
摘要
目的:探讨丰富环境对快速老化小鼠SAMP8情感的影响.方法:3 mo龄SAMP8小鼠和3 mo龄SAMRI小鼠随机分为丰富环境组(n=16)和标准环境组(n=10),饲养3 mo后用高架十字迷宫实验评测小鼠的情感改变.用RT-PCR方法检测小鼠海马脑源性神经生长因子(BDNF)的表达.结果:丰富环境组小鼠进入开放臂次数比例(OE%)及在开放臂停留的时间(OT%)比例均明显高于标准环境组(P<0.01).丰富环境组海马BDNF mRNA的表达明显高于标准环境组(P<0.01).结论:丰富环境能提高SAMP8小鼠海马BDNF mRNA的表达,并对SAMP8小鼠的精神行为具有干预作用,降低SAMP8小鼠的焦虑行为.
Abstract
AIM: To explore the effect of enriched environment on emotional behaviour in senescence accelerated-prone mice 8 (SAMP8). METHODS: Three-month female SAMP8 and 3-month female senescence accelerated-resistant mice1 (SAMR1) were divided into Enriched Environment (EE) group (n = 16) and Standard Environment (SE) group (n = 10) by random. After 3 months, the emotional behaviour was tested by Elevated Plus-Maze(EPM) and brain derived neurotrophic factor(BDNF) mRNA expression in hippocampus was tested by RT-PCR. RESULTS: EE mice had higher open arm entry percentage (OE%) and open arm time percentage(OT%) compared with SE mice (P ＜ 0.01). EE mice had higher BDNF mRNA expression in hippocampus compared with SE mice (P ＜ 0.01). CONCLUSION: EE could improve BDNF mRNA expression in hippocampus of SAMP8. EE also could change the emotional behaviour, which showed that EE could reduce the anxiety behaviour of SAMP8.
关键词
丰富环境/快速老化小鼠P8/高架十字迷宫/脑源性神经生长因子/情感Key words
enriched environment/SAMP8/elevated plusmaze/BDNF/emotion引用本文复制引用
基金项目
河北省自然科学基金(C2009001242)
出版年
2009
NETL
NSTL
维普
万方数据