Sero-reaction analysis and application of combinational detection of lung cancer candidate antigens
AIM:To investigate sero-reaction of 5 lung cancer candidate antigens which were identified by using SEREX(serological analysis of recombination cDNA expression libraries)and that of further combinational detection,to evaluate whether or not these antigens could be used as markers in early diagnosis of lung cancer and to provide a new direction of early diagnosis of lung cancer.METHODS:Five lung cancer candidate antigens(X5:partner of Ral-binding protein 1,POB1;X18:topisomerase [DNA]II alpha 170 kDa,Top2A;X44:tripeptidy 1 peptidase I,TPP1;X48:heat shock protein 90 kDa alpha,HSP90alpha;X52:eukaryotic translation elongation factor 1 gamma)were expressed from each corresponding cDNA clones and were subsequently used in immunoblotting analysis for detecting antibody responses with sera of lung cancer patients and normal human.The testing results were evaluated by epidemiological method.RESULTS:The positive rate of IgG antibodies of 3 antigens (POBl,Top2A and HSP90alpha)in lung cancer patients was obviously higher than that in normal controls;When the combinational analysis of these 3 antigen-antibody systems was performed,the sensitivity for detection of lung cancer was significantly increased with invariable specificity on the whole,suggesting that these 3 antigens may have a higher value to diagnose lung cancer early.CONCLUSION:This study can provide candidate markers in early diagnosis and combinational detection of lung cancer,and also highlight a new direction of early diagnosis of lung cancer.
serological analysis of recombination cDNA expression librarieslung tumorlung cancer associated antigenserological analysisearly diagnosis
NETL
NSTL
万方数据
维普
