Mechanism of heat shock-induced transcriptional inhibition of cyclinD1 expression
AIM:To investigate the heat shock response mechanism of human cyclin D1 promoter.METHODS:The mRNA level is detected by RT-PCR.The CAT reporter gene plasmids driven by the series of human cyclin D1 promoter truncations are constructed into pREP4-m-CAT vector.The responsible region is investigated by reporter gene analysis.The histone acetylation status is detected by ehromatin immunoprecipitation(ChIP).RESULTS:The mRNA level of cyclin D1 decreased under heat shock.This transcription depression is promoter activity dependent and the heat shock response region is located in-50-+141 region in which the Ac.H3k9 modification decreased.CONCLUSION:These results reveal that the heat inhibits cyclin D1 transcription involves its-50-+141 region.The histone acetylation modification decreased under heat treatment which may be responsible for the heat shock response.
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NSTL
万方数据
维普
