Effects of JNK signaling pathway on lipoapoptosis of βTc3 cells and the intervention of Pioglitazone
AIM:To investigate the effects of JNK signaling pathway on lipoapoptosis in mouse βTc3 cells and the intervention of pioglitazone(PGZ).METHODS:Free fatty acid(FFA)was used to induce the apoptosis of βTc3 cells.MTT assay was used to observe the inhibitory actions of FFA on βTc3 cells.The JNK inhibitor SP600125 and PGZ were used to protect βTc3 cells.The apoptotic rate of the cells was detected by flow eytometry.The expression of p-JNK,p-c-jun were detected by immunocytochemistry and Western Blot.RESULTS:The growth of the βTc3 cells were remarkably inhibited by FFA in vitro,and significant apoptosis was induced by FFA with the activation of p-JNK,p-c-jun.SP600125 and PGZ could inhibit the apoptosis significantly.But FFA-induced activation of p-JNK and p-c-jun was suppressed by the JNK inhibitor SP600125 but not PGZ.CONCLUSION:JNK signaling pathway was involved in lipoapoptosis of βTc3 cells.PGZ could inhibit the apoptosis of βTc3 cells stimulated by free fatty acids,but the JNK signaling pathway is not involved in the mechanism.
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