Effects of exendin-4 on expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in renal tissue of type 2 diabetic rats
AIM:To observe the effects of exendin-4 on the expression of matrix metalloproteinase-9(MMP-9)and tissue inhibitor of metalloproteinase-1(TIMP-1)in the renal tissue of type 2 diabetic rats.METHODS:The rat model of type 2 diabetes mellitus was set up by a combination of high fat diat and low dose streptozotocin(STZ).The diabetic rats were randomly divided into 4 groups:diabetes mellitus group(DM group),low dose of exendin-4 group(EL group),middle dose of exendin-4 group (EM group),high dose of exendin-4 group(EH group).Normal control group(NC group)was also established.After 6 weeks,the expression of MMP-9 and TIMP-1 in renal tissue was measured by immunohistochemistry.RESULTS:The expression of MMP-9 and TIMP-1 in DM group was higher than that in NC group(P＜0.01),while the expression of MMP-9 and TIMP-1 in all doses of exendin-4 groups was lower than that in DM group(P＜0.01).CONCLUSION:Exendin-4 has some renoprotective effect on type 2 diabetic rats,partly through down-regulating the expression of MMP-9 and TIMP-1.
exendin-4type 2 diabetes mellitusdiabetic nephropathymatrix metalloproteinase-9tissue inhibitor of metalloproteinase-1
NETL
NSTL
万方数据
维普
