Neuroprotective effect of emodin on rat brain tissues after cerebral blast injury
AIM:To observe the neuroprotective effects of emodin on the explosive blast injuried brain.METHODS:Seventy-eight adult SD rats were randomly divided into 3 groups:normal contrast group,model group,and emodin treatment group.There were 6 observational time points in each group:6,12 h,1,3,5,7 d with 6 rats in each time point.Cerebral blast injury models were produced except normal contrast group,and emodin was given 30 min after wounded[10 mg/(kg·d)intraperitoneal injection].After explosion,the breathes and behaviors of damaged rats were recorded.Levels of neuron specific enolase (NSE)in the serum and brain water content were measured at different time points.Meanwhile,the numbers of NSE immunohistochemical reactive cells were accounted in the cortex.RESULTS:The level of NSE in serum and brain water content of model group and emodin treatment group was obviously higher than pre-injury at most time points(P＜0.01),and it reached the peak at 24 h after injury,then,it began to decrease,and still higher than pre-injury at 5 d.The number of NSE immunohistochemical reactive cells in the cortex was significantly decreased.The emodin group has a similar tendency compare with the control group but had an earlier peak time at 12 h after injury and reached normal level at 3 d after injury.CONCLUSION:Emodin presents protective effects on wounded brain in rats with cerebral blast injury.
emodincerebral blast injuryneuron specific enolase
NETL
NSTL
万方数据
维普
